Arbutin is a potential depigmenting agent that reduces melanin production by inhibiting tyrosinase activity and predicts the risk of whitening of the skin. Despite wide acceptance in routine laboratory tests, the conventional methods for whitening agent analysis have been of limited use because of high cost and lower signal sensitivity. Gold nanoparticles have gained considerable interest in improving the sensitivity and signals for the detection of proteins. Inspiring from these strategies, we have designed a tyrosinase enzyme gold nanoparticles (tyrosinase–AuNPs) method for the evaluation of arbutin with improved signals. The developed assay has been tested for tyrosine spiked samples to derive the standard graph. And then the performance of the assay is validated by testing arbutin on inhibiting tyrosinase activity. A detection limit of 13.70 μM with R² of 0.982 is confirmed. Compared with the conventional method, the proposed assay represented the improved sensitivity, and signals are enhanced by five times that can be easily visualized through the naked eyes. Moreover, our method is beneficial because of the low cost and avoiding the need for expensive instruments. We believe that this method for arbutin evaluation will provide a foundation for the screening of other whitening agents, paving the way for future advances in the field of cosmetics.

中文翻译：

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使用基于酪氨酸酶金纳米颗粒的比色测定法监测用于皮肤分析的增白剂

熊果苷是一种潜在的脱色剂，可通过抑制酪氨酸酶活性来减少黑色素的产生，并预测皮肤变白的风险。尽管在常规实验室测试中被广泛接受，但是用于白化剂分析的常规方法由于成本高和信号灵敏度低而受到限制。金纳米颗粒已对改善用于检测蛋白质的灵敏度和信号引起了极大的兴趣。从这些策略中得到启发，我们设计了一种酪氨酸酶金纳米颗粒（酪氨酸酶–AuNPs）方法，用于评估具有改善信号的熊果素。已开发的测定方法已测试了酪氨酸加标样品，以得出标准图。然后通过测试熊果苷对酪氨酸酶活性的抑制来验证该测定的性能。检出限为13。确认R ²为0.982。与传统方法相比，所提出的测定方法具有更高的灵敏度，并且信号可以增强五倍，可以通过肉眼轻松看到。此外，我们的方法是有益的，因为其成本低并且避免了对昂贵仪器的需求。我们认为，这种熊果苷评估方法将为筛选其他增白剂提供基础，为化妆品领域的未来发展铺平道路。