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A tomato dynein light chain gene SlLC6D is a negative regulator of chilling stress
Plant Science ( IF 5.2 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.plantsci.2020.110753
Tixu Hu , Shufeng Wang , Qi Wang , Xin Xu , Qiqi Wang , Xiangqiang Zhan

Dynein light chain (DLC) proteins are an important component of dynein complexes, which are widely distributed in plants and animals and involved in a variety of cellular processes. The functions of DLC genes in plant chilling stress remain unclear. In this study, we isolated a DLC gene from tomato, designated SlLC6D. Promoter analysis revealed many cis-elements involved in abiotic stress in the SlLC6D promoter. Expression of SlLC6D was induced by heat and salt stress, and inhibited by polyethylene glycol and chilling stress. Knockdown of SlLC6D in tomato exhibited low relative electrolyte leakage, malondialdehyde content, and reactive oxygen species (ROS) accumulation under chilling stress. The content of proline and activities of superoxide dismutase and peroxidase in knockdown lines were higher than in the wild type and overexpression lines during chilling stress. The high transcript abundances of three cold-responsive genes were detected in knockdown lines in response to chilling stress. Seedling growth of knockdown lines was significantly higher than that of the wild type and overexpression lines under chilling stress. These results suggest that SlLC6D is a negative regulator of chilling stress tolerance, possibly by regulating ROS contents and the ICE1-CBF-COR pathway.

中文翻译:

番茄动力蛋白轻链基因 SlLC6D 是低温胁迫的负调节因子

动力蛋白轻链(DLC)蛋白是动力蛋白复合物的重要组成部分,广泛分布于动植物中,参与多种细胞过程。DLC 基因在植物低温胁迫中的功能尚不清楚。在这项研究中,我们从番茄中分离出一个 DLC 基因,命名为 SlLC6D。启动子分析揭示了 SlLC6D 启动子中涉及非生物胁迫的许多顺式元件。SlLC6D 的表达受热和盐胁迫诱导,并受聚乙二醇和冷胁迫抑制。在低温胁迫下,番茄中 SlLC6D 的敲除表现出较低的相对电解质泄漏、丙二醛含量和活性氧 (ROS) 积累。低温胁迫下敲除系脯氨酸含量及超氧化物歧化酶和过氧化物酶活性均高于野生型和过表达系。在响应寒冷胁迫的敲低系中检测到三个冷响应基因的高转录本丰度。在低温胁迫下,敲除系的幼苗生长显着高于野生型和过表达系的幼苗生长。这些结果表明 SlLC6D 是寒冷胁迫耐受性的负调节因子,可能是通过调节 ROS 含量和 ICE1-CBF-COR 途径。在低温胁迫下,敲除系的幼苗生长显着高于野生型和过表达系的幼苗生长。这些结果表明 SlLC6D 是寒冷胁迫耐受性的负调节因子,可能是通过调节 ROS 含量和 ICE1-CBF-COR 途径。在低温胁迫下,敲除系的幼苗生长显着高于野生型和过表达系的幼苗生长。这些结果表明 SlLC6D 是寒冷胁迫耐受性的负调节因子,可能是通过调节 ROS 含量和 ICE1-CBF-COR 途径。
更新日期:2021-02-01
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