Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-11-18 , DOI: 10.1016/j.colsurfb.2020.111473 Bolin Tang 1 , Xiaojun Shen 1 , Yaru Yang 1 , Zhi Xu 1 , Jie Yi 1 , Yongbo Yao 1 , Miao Cao 1 , Yalin Zhang 2 , Hongqin Xia 2
Cellular responses can be regulated and manipulated through combining stimuli-responsive biomaterial with external stimulus. In this present, the magneto-responsive CoFe2O4/P(VDF-TrFE) nanocomposite coatings were designed to understand cell behaviors of preosteoblasts, as well as get insight into the underlying mechanism of osteogenic differentiation under static magnetic field (SMF). CoFe2O4/P(VDF-TrFE) nanocomposite coatings with differential magnetic property (low, medium and high magnetization) were prepared by incorporation of different mass fraction of CoFe2O4 nanoparticles (6%, 13%, 20%) into P(VDF-TrFE) matrix. Cell experiments indicated that all nanocomposite coatings with the assistance of SMF could promote the cell attachment, proliferation and osteogenic differentiation of MC3T3-E1 cells. Among different nanocomposite coatings, low magnetization coating (6%) showed a higher ALP activity and gene expression of Runx2, Col-I, OCN. Molecular biology assays demonstrated that the combination of nanocomposite coatings and SMF could significantly up-regulate the expression level of α2β1 integrin and p-ERK. Whereas, the addition of inhibitor U0126 down-regulated sharply the expression level of p-ERK, which indicated that cellular osteogenic differentiation of MC3T3-E1 cells was governed through α2β1 integrin-mediated MEK/ERK signaling pathways during CoFe2O4/P(VDF-TrFE) nanocomposite coatings were combined with SMF. This work provided a promising strategy to enhance cellular osteogenic differentiation through a remote-control manner, which exhibited great potential in the application of bone tissue repair and regeneration.
中文翻译:
静态磁场下CoFe 2 O 4 / P(VDF-TrFE)纳米复合涂层的细胞成骨分化增强
通过将刺激响应性生物材料与外部刺激相结合,可以调节和操纵细胞反应。在目前,磁响应CoFe 2 O 4 / P(VDF-TrFE)纳米复合涂层旨在了解成骨细胞的细胞行为,并深入了解在静磁场(SMF)下成骨分化的潜在机制。通过掺入不同质量分数的CoFe 2 O 4,制备了具有不同磁性(低,中,高磁化强度)的CoFe 2 O 4 / P(VDF-TrFE)纳米复合涂层。纳米颗粒(6%,13%,20%)放入P(VDF-TrFE)基质中。细胞实验表明,在SMF的帮助下,所有纳米复合材料涂层都可以促进MC3T3-E1细胞的附着,增殖和成骨分化。在不同的纳米复合涂层中,低磁化涂层(6%)显示出更高的ALP活性和Runx2,Col-1,OCN的基因表达。分子生物学分析表明,纳米复合涂层和SMF的结合可以显着上调α2β1整联蛋白和p-ERK的表达水平。而抑制剂U0126的加入则下调了p-ERK的表达水平,这表明MC3T3-E1细胞的成骨分化是通过CoFe 2 O期间的α2β1整合素介导的MEK / ERK信号通路来控制的。将4 / P(VDF-TrFE)纳米复合涂层与SMF混合。这项工作提供了一种有希望的策略,可以通过远程控制的方式来增强细胞的成骨分化,这在骨组织修复和再生的应用中显示出巨大的潜力。