In this study, we utilized a carboxyalkylphosphonic acid covered fluorine doped tin oxide (FTO) as an electrode material for fabrication of Interleukin 1α (IL-1α) immunosensor. For this aim, anti-IL-1α antibodies were attached on the 3-phosphonopropionic acid (PHP) modified FTO surface covalently. Electrochemical (electrochemical impedance spectroscopy and cyclic voltammetry) and morphological (scanning electron microscopy and atomic force microscopy) characterizations were performed to monitor the successful fabrication of immunoelectrodes. After incubation of anti-IL-1α antibody immobilized FTO electrodes in IL-1α antigen solutions, increases were seen in impedimetric responses. IL-1α antigen was determined in a linear detection range from 0.02 to 2 pg/mL by EIS. The detection limit of the suggested immunosensor was 6 fg/mL. The applicability of the designed biosensor was tested by using human serum and saliva samples and acceptable results were obtained. In addition, high sensitivity, good specificity, low detection limit made the proposed immunosensor a potential technique for IL-1α antigen determination in routine clinical analysis.

在这项研究中，我们利用羧基烷基膦酸覆盖的氟掺杂氧化锡（FTO）作为电极材料制造白介素1α（IL-1α）免疫传感器。为了这个目的，将抗IL-1α抗体共价连接到3-膦酰基丙酸（PHP）修饰的FTO表面上。进行了电化学（电化学阻抗谱和循环伏安法）和形态学（扫描电子显微镜和原子力显微镜）表征，以监测免疫电极的成功制造。在IL-1α抗原溶液中孵育固定有抗IL-1α抗体的FTO电极后，在阻抗反应中可见增加。通过EIS在0.02至2 pg / mL的线性检测范围内确定IL-1α抗原。建议的免疫传感器的检出限为6 fg / mL。通过使用人血清和唾液样品测试设计的生物传感器的适用性，并获得可接受的结果。此外，高灵敏度，特异性好，检测限低等特点使该免疫传感器成为常规临床分析中IL-1α抗原测定的潜在技术。