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Detection of a novel glycodelin biomarker using electrochemical immunosensor for endometriosis
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.aca.2020.11.015
Thangapandi Kalyani , Amalesh Nanda , Saikat Kumar Jana

Endometriosis is one of the important issues in women worldwide, which decreases the quality of women's lives in their reproductive age. The diagnosis of endometriosis is carried out by the invasive procedure, which is expensive and painful. In the last few decades, researchers have given more attention to constructing a suitable biomarker-based biosensor for semi/non-invasive diagnosis of endometriosis. As a result, glycodelin (GLY) was found as a promising biomarker because of its selectivity and sensitivity. To the best of our knowledge, it was the first study that reported the detection of GLY biomarker using an electrochemical immunosensor. Briefly, a label-free electrochemical immunosensing platform was constructed through in-situ surface modification of cysteamine layer and immobilisation of antibody (anti-GLY) with help of glutaraldehyde. The interaction between antigen and antibody was measured using square wave voltammetry (SWV). The SWV signal could decrease proportionally with the increasing GLY concentration ranging from 1 to 1000 ng mL-1 (R2 = 0.9981) and a detection limit (LOD) of 0.43 ng mL-1. Moreover, an immunosensor could exhibit high sensitivity, selectivity, long-term stability, reproducibility and regeneration. Accuracy of the immunosensor was compared with enzyme-linked immunosorbent assay (ELISA), and satisfying results were obtained. The detection of GLY biomarker may be a new possibility for endometriosis diagnosis.

中文翻译:

使用电化学免疫传感器检测子宫内膜异位症的新型多糖蛋白生物标志物

子宫内膜异位症是全球女性面临的重要问题之一,它降低了育龄妇女的生活质量。子宫内膜异位症的诊断是通过侵入性手术进行的,这是昂贵且痛苦的。在过去的几十年中,研究人员更加关注构建合适的基于生物标志物的生物传感器,用于子宫内膜异位症的半/非侵入性诊断。因此,糖苷脂 (GLY) 因其选择性和灵敏度而被认为是一种很有前途的生物标志物。据我们所知,这是第一项报道使用电化学免疫传感器检测 GLY 生物标志物的研究。简要地,通过半胱胺层的原位表面修饰和戊二醛的帮助下抗体(抗GLY)的固定,构建了无标记电化学免疫传感平台。使用方波伏安法(SWV)测量抗原和抗体之间的相互作用。SWV 信号可以随着 GLY 浓度从 1 到 1000 ng mL-1 (R2 = 0.9981) 和 0.43 ng mL-1 的检测限 (LOD) 的增加而成比例地降低。此外,免疫传感器可以表现出高灵敏度、选择性、长期稳定性、重现性和再生性。将免疫传感器的准确度与酶联免疫吸附试验(ELISA)进行了比较,得到了满意的结果。GLY生物标志物的检测可能为子宫内膜异位症的诊断提供新的可能性。使用方波伏安法(SWV)测量抗原和抗体之间的相互作用。SWV 信号可以随着 GLY 浓度从 1 到 1000 ng mL-1 (R2 = 0.9981) 和 0.43 ng mL-1 的检测限 (LOD) 的增加而成比例地降低。此外,免疫传感器可以表现出高灵敏度、选择性、长期稳定性、重现性和再生性。将免疫传感器的准确度与酶联免疫吸附试验(ELISA)进行了比较,得到了满意的结果。GLY生物标志物的检测可能为子宫内膜异位症的诊断提供新的可能性。使用方波伏安法(SWV)测量抗原和抗体之间的相互作用。SWV 信号可以随着 GLY 浓度从 1 到 1000 ng mL-1 (R2 = 0.9981) 和 0.43 ng mL-1 的检测限 (LOD) 的增加而成比例地降低。此外,免疫传感器可以表现出高灵敏度、选择性、长期稳定性、重现性和再生性。将免疫传感器的准确度与酶联免疫吸附试验(ELISA)进行了比较,得到了满意的结果。GLY生物标志物的检测可能为子宫内膜异位症的诊断提供新的可能性。免疫传感器可以表现出高灵敏度、选择性、长期稳定性、重现性和再生性。将免疫传感器的准确度与酶联免疫吸附试验(ELISA)进行了比较,得到了满意的结果。GLY生物标志物的检测可能为子宫内膜异位症的诊断提供新的可能性。免疫传感器可以表现出高灵敏度、选择性、长期稳定性、重现性和再生性。将免疫传感器的准确度与酶联免疫吸附试验(ELISA)进行了比较,得到了满意的结果。GLY生物标志物的检测可能为子宫内膜异位症诊断提供新的可能性。
更新日期:2021-02-01
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