This study investigates the decolorization potential of actinobacteria from soil towards toxic triphenylmethane (TPM) dyes, i.e., malachite green (MG), methyl violet (MV), crystal violet (CV), and cotton blue (CB). The actinobacterial isolates were first isolated from fresh soil samples, plated onto actinomycetes isolation agar (AIA), and both live and dead cells were prepared to evaluate their decolorization efficiency (DE). Isolates with positive decolorization activities were identified via partial sequencing of the 16S rRNA region. They were revealed as species of Nocardiopsis (N. alba), Streptomyces (S. puniceus, S. bacillaris, S. albolongus, S. acidiscabies, S. albulus, S. pratensis, S. luridiscabiei, S. rubiginosus, S. albidochromogenes), Rhodococcus (R. sovatensis), and Kitasatospora (K. albolonga). Results indicated that all 12 actinobacterial strains (live cells and dead cells) were able to decolorize TPM dyes, although with varying degree of effectiveness. Isolate N. alba (live cells) achieved the highest DE, with 97.0, 95.1, 95.8, and 83.8% (day 14) for MG, MV, CV, and CB, respectively. This was followed by live cells of S. bacillaris with 94.7, 95.1, 90.5, and 63.9% of DE for the same dyes. Live cells appeared to be more effective in decolorizing TPM dyes, suggesting the possible biosorption and biodegradation of dyes. It is concluded that soil actinobacteria tested in this study have the potential for removal of TPM dyes.

这项研究调查了放线菌从土壤向有毒三苯甲烷（TPM）染料（即孔雀石绿（MG），甲基紫（MV），结晶紫（CV）和棉蓝（CB））的脱色潜力。首先从新鲜的土壤样品中分离出放线菌的分离株，将其放到放线菌分离琼脂（AIA）上，准备活细胞和死细胞以评估其脱色效率（DE）。通过对16S rRNA区进行部分测序，鉴定出具有正脱色活性的分离株。调查发现她们的物种诺卡（N.阿尔巴），链霉菌（S.鲜红，S. bacillaris，S. albolongus，S. acidiscabies，S. albulus，S.禾，S. luridiscabiei，S. rubiginosus，S. albidochromogenes），红球菌属（R. sovatensis），和Kitasatospora（K. albolonga）。结果表明，所有十二种放线菌菌株（活细胞和死细胞）均能使TPM染料脱色，尽管其效果不同。分离的白杆菌（活细胞）获得了最高的DE，MG，MV，CV和CB分别为97.0、95.1、95.8和83.8％（第14天）。其次是杆菌链球菌的活细胞相同染料的DE含量为94.7％，95.1％，90.5％和63.9％。活细胞似乎在使TPM染料脱色方面更有效，表明该染料可能具有生物吸附作用和生物降解作用。结论是，在这项研究中测试的土壤放线菌具有去除TPM染料的潜力。