This study was conducted to examine the effect of formulated resuscitation-promoting broths on the revival of viable but nonculturable Vibrio parahaemolyticus induced by cold and starvation stresses. Vibrio parahaemolyticus was incubated in artificial sea water at 4 °C for more than 8 months until this bacterium became undetectable, while retaining its intact cell count of more than 10⁵ CFU/field over time. On day 250, V. parahaemolyticus was collected and enriched in tryptic soy broth supplemented with 3% NaCl, 10,000 U/mg catalase, 2% sodium pyruvate, 20 mM MgSO₄, 5 mM EDTA, and a cell-free supernatant taken from V. parahaemolyticus ATCC 17802 in the stationary phase (pH 8). V. parahaemolyticus returned partially to a culturable state with a maximal cell density of 7.91 log CFU/mL in this formulated medium following 7 days of enrichment at 25 °C. In contrast, no V. parahaemolyticus was resuscitated when enriched in alkaline peptone water and tryptic soy broth.

进行这项研究是为了检查配制的复苏促进肉汤对寒冷和饥饿应激诱导的有活力但不可培养的副溶血性弧菌复苏的影响。副溶血性弧菌在 4 °C 的人工海水中培养 8 个月以上，直到这种细菌变得无法检测到，同时 随着时间的推移保持其完整的细胞计数超过 10 ⁵ CFU/场。在第 250 天，V。收集副溶血性菌并在补充有 3% NaCl、10,000 U/mg 过氧化氢酶、2% 丙酮酸钠、20 mM MgSO ₄、5 mM EDTA 和从副溶血性弧菌中提取的无细胞上清液的胰蛋白酶大豆肉汤中富集固定相 (pH 8) 中的 ATCC 17802。在 25 °C 下富集 7 天后，副溶血性弧菌在该配制培养基中部分恢复到可培养状态，最大细胞密度为 7.91 log CFU/mL。相比之下，没有V。当富含碱性蛋白胨水和胰蛋白酶大豆肉汤时，副溶血性杆菌被复苏。