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RNA partitioning into stress granules is based on the summation of multiple interactions
RNA ( IF 4.5 ) Pub Date : 2020-11-16 , DOI: 10.1261/rna.078204.120
Tyler Matheny 1 , Briana Van Treeck 1 , Thao Ngoc Huynh 1 , Roy Parker 1, 2
Affiliation  

Stress granules (SGs) are stress-induced RNA-protein assemblies formed from a complex transcriptome of untranslating ribonucleoproteins (RNPs). Although RNAs can be either enriched or depleted from SGs, the rules that dictate RNA partitioning into SGs are unknown. We demonstrate that the SG-enriched NORAD RNA is sufficient to enrich a reporter RNA within SGs through the combined effects of multiple elements. Moreover, artificial tethering of G3BP1, TIA1, or FMRP can target mRNAs into SGs in a dose-dependent manner with numerous interactions required for efficient SG partitioning, which suggests individual protein interactions have small effects on the SG partitioning of mRNPs. This is supported by the observation that the SG transcriptome is largely unchanged in cell lines lacking the abundant SG RNA-binding proteins G3BP1 and G3BP2. We suggest the targeting of RNPs into SGs is due to a summation of potential RNA-protein, protein-protein, and RNA-RNA interactions with no single interaction dominating RNP recruitment into SGs.

中文翻译:

RNA 分配成应激颗粒是基于多重相互作用的总和

应激颗粒 (SG) 是应激诱导的 RNA 蛋白组装体,由非翻译核糖核蛋白 (RNP) 的复杂转录组形成。尽管 RNA 可以从 SG 中富集或耗尽,但决定 RNA 分配到 SG 中的规则尚不清楚。我们证明,通过多种元素的综合作用,富含 SG 的 NORAD RNA 足以富集 SG 内的报告 RNA。此外,G3BP1、TIA1 或 FMRP 的人工束缚可以以剂量依赖性方式将 mRNA 靶向 SG,并具有有效 SG 分配所需的大量相互作用,这表明单个蛋白质相互作用对 mRNP 的 SG 分配影响很小。观察结果支持了这一点,即在缺乏丰富的 SG RNA 结合蛋白 G3BP1 和 G3BP2 的细胞系中,SG 转录组基本上没有变化。我们认为,RNP 靶向 SG 是由于潜在的 RNA-蛋白质、蛋白质-蛋白质和 RNA-RNA 相互作用的总和,没有单一相互作用主导 RNP 募集到 SG 中。
更新日期:2020-11-16
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