Objectives. To observe the effect of Inonotus obliquus polysaccharide (IOP) on the proliferation, invasion, migration, and apoptosis of osteosarcoma cells and to elucidate its underlying molecular mechanism. Methods. IOP was extracted from Inonotus obliquus, human osteosarcoma MG-63 cells and U2OS cells were cultured in vitro, and the effects of IOP on the proliferation, migration, invasion, and apoptosis of MG-63 cells and U2OS cells were determined by CCK-8 assays, cell scratch assays, transwell assays, and flow cytometry, respectively. Western blot was used to detect the expression of related proteins in the Akt/mTOR and NF-κB signaling pathways. Results. Compared with the control group, MG-63 cells and U2OS cells treated with IOP of 80 μg/ml, 160 μg/ml, and 320 μ g/ml in the experimental group had significantly lower proliferation activity, decreased migration and invasion ability, and increased apoptosis rate (). Furthermore, IOP could significantly inhibit the activation of the Akt/mTOR and NF-κB signaling pathway (). Conclusion. IOP can regulate the proliferation, migration, invasion, and apoptosis of osteosarcoma cells by inhibiting the activation of the Akt/mTOR signaling pathway. It has antitumor activity on osteosarcoma and has the potential of clinical application in osteosarcoma treatment.

中文翻译：

---

桦褐孔菌多糖对骨肉瘤细胞增殖、侵袭、迁移和凋亡的影响

目标。观察桦褐孔菌多糖（IOP）对骨肉瘤细胞增殖、侵袭、迁移和凋亡的影响，阐明其潜在分子机制。方法。从桦褐孔菌中提取眼压，体外培养人骨肉瘤MG-63细胞和U2OS细胞，CCK-8测定眼压对MG-63和U2OS细胞增殖、迁移、侵袭和凋亡的影响。分析、细胞划痕分析、transwell 分析和流式细胞术。Western印迹用于检测Akt/mTOR和NF-κB信号通路中相关蛋白的表达。结果. 与对照组相比，实验组在IOP分别为80  μ g/ml、160  μ g/ml和320  μ  g/ml的MG-63细胞和U2OS细胞增殖活性明显降低，迁移和侵袭能力下降。 , 和增加的细胞凋亡率 (）。此外，IOP 可显着抑制 Akt/mTOR 和 NF-κB 信号通路的激活（）。 结论。IOP可以通过抑制Akt/mTOR信号通路的激活来调节骨肉瘤细胞的增殖、迁移、侵袭和凋亡。对骨肉瘤具有抗肿瘤活性，在骨肉瘤治疗中具有临床应用潜力。