The Twort-like myoviruses (Kayvirus genus) of S. aureus are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the Kayvirus genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp).

中文翻译：

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金黄色葡萄球菌巨噬病毒噬菌体vB_SauM-515A1的转录景观

由于宿主范围广且对临床分离株具有高杀灭活性，金黄色葡萄球菌的Twort -like肌病毒（花王病毒属）是噬菌体治疗的有前途的药物。这项工作通过转录组分析提高了目前对噬菌体感染生理的理解。典型的山猫病毒成员的表达谱属（vB_SauM-515A1）在感染后三个时间点使用RNA测序获得。建立了总共35个包含238个ORF的转录单位。在噬菌体基因组中鉴定了58个早期和12个晚期启动子的序列。早期启动子代表强大的sigma-70启动子共有序列，并控制26个转录单位（占基因的81％）的宿主依赖性表达。晚期启动子专门控制四个转录单元的表达，而其他五个单元的转录则由两种类型的启动子指导。晚期启动子的特征是TGTTATATTA共有序列长-10个框，缺少-35个框。获得的数据也具有普遍意义，