ABSTRACT

Mansoa hymenaea (DC.) A.H. Gentry is used in traditional medicine to treat pain, arthritis, and rheumatoid disease. It has also been demonstrated that species belonging to the Mansoa genus exert cytotoxic activity. Despite this data, biological properties and phytochemistry of M. hymenaea remain poorly understood. Therefore, we determined the metabolite profile of the methanolic extract of M. hymenaea leaves and evaluated its anti-inflammatory, antinociceptive, and cytotoxic activity. Phytochemical analysis was carried out by conventional methods and gas chromatography-mass spectrometry. Anti-inflammatory and antinociceptive effects were assessed in the TPA‑induced ear edema model and acetic acid-induced writhing test, respectively. Cytotoxic activity was determined in vitro against SW-620, MDA-MB231, SiHa, SKLU1, and PC-3 cancer cell lines. The results revealed that M. hymenaea methanolic extract at 2 mg/ear reduced inflammation significantly (p ≤ 0.05) by 50.4 ± 5.9 %, and decreased visceral pain with an effective dose 50 (ED₅₀) of 148.9 ± 14.1 mg kg⁻¹. Moreover, M. hymenaea exerted cytotoxic activity against the breast carcinoma cell line (MDA-MB231) with IC₅₀ = 125.8 ± 16.7 µg mL⁻¹. Chemical analyses revealed that M. hymenaea methanolic extract contained alkaloids, glycosides, tannins, sterols, and saponins. Likewise, trans-13-octadecenoic acid, n-hexadecanoic acid, diallyl disulfide, diallyl trisulfide, β-sitosterol, methyl ursolate, stigmasterol, and phytol were detected by CG-MS in the M. hymenaea methanolic extract. These compounds were previously reported with anti-inflammatory, antinociceptive, or cytotoxic effects. Therefore, the methanolic extract of M. hymenaea leaves is a good source of natural compounds with anti-inflammatory, antinociceptive, and cytotoxic properties.

抽象的

Mansoa hymenaea（DC。）AH Gentry在传统医学中用于治疗疼痛，关节炎和类风湿病。还已经证明，属于Mansoa属的物种发挥细胞毒活性。尽管有这些数据，但对膜状支原体的生物学特性和植物化学仍然知之甚少。因此，我们确定了膜状支原体甲醇提取物的代谢物谱。离开并评估其抗炎，抗伤害感受和细胞毒性活性。通过常规方法和气相色谱-质谱法进行植物化学分析。在TPA引起的耳部水肿模型和乙酸引起的扭体试验中分别评估了抗炎和镇痛作用。在体外确定了针对SW-620，MDA-MB231，SiHa，SKLU1和PC-3癌细胞系的细胞毒活性。结果表明，2 mg / ear的膜状分枝杆菌甲醇提取物可显着降低炎症（p≤0.05）50.4±5.9％，有效剂量50（ED ₅₀）为148.9±14.1 mg kg ^-1可减少内脏疼痛。此外，猪笼草对乳腺癌细胞系（MDA-MB231）产生细胞毒活性，IC ₅₀  = 125.8±16.7 µg mL ^-1。化学分析显示，膜状支原体甲醇提取物含有生物碱，糖苷，单宁，固醇和皂苷。同样地，通过CG-MS在膜膜分枝杆菌甲醇提取物中检测到了反式13-十八碳烯酸，正十六烷酸，二烯丙基二硫化物，二烯丙基三硫化物，β-谷甾醇，尿酸甲酯，豆甾醇和植物甾醇。先前已报道这些化合物具有抗炎，抗伤害或细胞毒性作用。因此，猪笼草的甲醇提取物 叶子是具有抗炎，抗伤害和细胞毒性特性的天然化合物的良好来源。