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Electrolocation? The evidence for redox-mediated taxis in Shewanella oneidensis
Molecular Microbiology ( IF 3.6 ) Pub Date : 2020-11-17 , DOI: 10.1111/mmi.14647
Ruth Starwalt-Lee 1 , Mohamed Y El-Naggar 2, 3, 4 , Daniel R Bond 1 , Jeffrey A Gralnick 1
Affiliation  

Shewanella oneidensis is a dissimilatory metal reducing bacterium and model for extracellular electron transfer (EET), a respiratory mechanism in which electrons are transferred out of the cell. In the last 10 years, migration to insoluble electron acceptors for EET has been shown to be nonrandom and tactic, seemingly in the absence of molecular or energy gradients that typically allow for taxis. As the ability to sense, locate, and respire electrodes has applications in bioelectrochemical technology, a better understanding of taxis in S. oneidensis is needed. While the EET conduits of S. oneidensis have been studied extensively, its taxis pathways and their interplay with EET are not yet understood, making investigation into taxis phenomena nontrivial. Since S. oneidensis is a member of an EET-encoding clade, the genetic circuitry of taxis to insoluble acceptors may be conserved. We performed a bioinformatic analysis of Shewanella genomes to identify S. oneidensis chemotaxis orthologs conserved in the genus. In addition to the previously reported core chemotaxis gene cluster, we identify several other conserved proteins in the taxis signaling pathway. We present the current evidence for the two proposed models of EET taxis, “electrokinesis” and flavin-mediated taxis, and highlight key areas in need of further investigation.

中文翻译:

电定位?Shewanella oneidensis 中氧化还原介导的出租车的证据

Shewanella oneidensis是一种异化金属还原细菌和细胞外电子转移 (EET) 模型,EET 是一种将电子转移出细胞的呼吸机制。在过去的 10 年中,EET 向不溶性电子受体的迁移已被证明是非随机和有策略的,似乎没有通常允许出租车的分子或能量梯度。由于感应、定位和呼吸电极的能力已在生物电化学技术中得到应用,因​​此需要更好地了解奥奈德斯的出租车。虽然S. oneidensis的 EET 管道已被广泛研究,但其出租车路径及其与 EET 的相互作用尚不清楚,因此对出租车现象的调查并非易事。自从S. oneidensis是 EET 编码进化枝的成员,出租车到不溶性受体的遗传电路可能是保守的。我们对希瓦氏菌基因组进行了生物信息学分析,以确定该属中保守的S. oneidensis趋化性直向同源物。除了先前报道的核心趋化基因簇外,我们还鉴定了出租车信号通路中的其他几种保守蛋白质。我们展示了两种拟议的 EET 出租车模型的当前证据,即“电动”和黄素介导的出租车,并强调了需要进一步调查的关键领域。
更新日期:2020-11-17
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