This experiment mainly optimized the extraction technology of Agaricus blazei polypeptide (ABp) and evaluated its protective effect on aging mice. In this study, a novel single component, the M is 3 kD, was isolated and purified from Agaricus blazei. An aging mouse model was established using D‐galactose. After the administration of ABp, the contents of total antioxidant capacity (T‐AOC), malondialdehyde (MDA), catalase (CAT), and reactive oxygen species were significantly changed. Through immunofluorescence staining, it was observed that ABp can reduce changes in brain tissue. The differential expression of genes was analyzed by RNA‐seq. A total of 295 differentially expressed genes were screened out in the ABp group.RT‐qPCR verified important genes and showed that the mRNA expression levels of Hsph1, Trim32, HK1, Hnrnpa1, and Grik5 were significantly increased, and those of ApoE, Atp1a3, Stxbp1, and Mapk8ip1 was significantly decreased. Western blotting showed that the protein expression levels of Keap1 and p53 were significantly lower, while the protein expression levels of Nrf2, HO‐1, Hsph1, and Trim32 were significantly higher in the ABP group. ABp played an anti‐aging role in an aging mouse model. The specific mechanism of action may be related to the regulation of the expression of the Keap1/Nrf2/P53 signaling pathway and related factors.

中文翻译：

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姬松茸多肽通过 Keap1/Nrf2/ARE 和 P53/Trim32 信号通路对 D-半乳糖诱导的衰老小鼠发挥保护作用

本实验主要优化姬松茸多肽（ABp）的提取工艺，并评价其对衰老小鼠的保护作用。在这项研究中，从姬松茸中分离纯化了一种新的单组分 M 为 3 kD。使用D-半乳糖建立衰老小鼠模型。ABp给药后，总抗氧化能力（T-AOC）、丙二醛（MDA）、过氧化氢酶（CAT）和活性氧的含量发生了显着变化。通过免疫荧光染色，观察到ABp可以减少脑组织的变化。通过RNA-seq分析基因的差异表达。ABp组共筛选出295个差异表达基因。RT-qPCR验证重要基因，显示Hsph1、Trim32、HK1、Hnrnpa1、和Grik5显着升高，ApoE、Atp1a3、Stxbp1和Mapk8ip1显着降低。Western blotting显示ABP组Keap1和p53蛋白表达水平显着降低，而Nrf2、HO-1、Hsph1和Trim32蛋白表达水平显着升高。ABp 在衰老小鼠模型中发挥抗衰老作用。具体作用机制可能与调控Keap1/Nrf2/P53信号通路的表达及相关因素有关。ABp 在衰老小鼠模型中发挥抗衰老作用。具体作用机制可能与调控Keap1/Nrf2/P53信号通路的表达及相关因素有关。ABp 在衰老小鼠模型中发挥抗衰老作用。具体作用机制可能与调控Keap1/Nrf2/P53信号通路的表达及相关因素有关。