We firstly identified 48 kDa molecular form of the unconventional myosin 1c (p48/Myo1C), and isolated it from blood serum of multiple sclerosis patients. The amount of p48/Myo1C in human blood serum correlated with some autoimmune, hemato‐oncological and neurodegenerative diseases and thus may serve as a potential molecular biomarker. The biological functions of this protein in human blood remain unknown. Previously, we used the monodisperse magnetic poly (glycidyl methacrylate)(mag‐PGMA–NH₂) microspheres with immobilized 48/Myo1C and western‐blot analysis, which allowed us to identify IgM and IgG immunoglobulins presenting an affinity to this protein. Here, we used mass spectrometry followed by the western blotting in order to identify other blood serum proteins with affinity to 48/Myo1C. The obtained data demonstrate that 48/Myo1C binds to component 3 of the complement and the antithrombin‐III proteins. A combination of magnetic microparticle‐based affinity chromatography with MALDI–TOF mass spectrometry and an in silico analysis provided an opportunity to identify the partners of interaction of 48/Myo1C with other proteins, in particular those participating in complement and coagulation cascades.

中文翻译：

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具有与48 kDa非常规肌球蛋白1c结合能力的蛋白在人血清中的分离和鉴定及其可能的诊断和预后价值

我们首先确定了非常规肌球蛋白1c（p48 / Myo1C）的48 kDa分子形式，并将其从多发性硬化症患者的血清中分离出来。人血清中p48 / Myo1C的含量与某些自身免疫性疾病，血液肿瘤学和神经退行性疾病有关，因此可能作为潜在的分子生物标志物。该蛋白在人血中的生物学功能仍然未知。以前，我们使用单分散磁性聚（甲基丙烯酸缩水甘油酯）（mag-PGMA-NH ₂）固定化48 / Myo1C的微球并进行Western印迹分析，这使我们能够鉴定出对该蛋白具有亲和力的IgM和IgG免疫球蛋白。在这里，我们使用质谱分析，然后进行蛋白质印迹分析，以鉴定对48 / Myo1C具有亲和力的其他血清蛋白。获得的数据表明48 / Myo1C与补体的成分3和抗凝血酶III蛋白结合。基于磁性微粒的亲和色谱与MALDI-TOF质谱以及计算机分析相结合，提供了机会来确定48 / Myo1C与其他蛋白质（尤其是参与补体和凝血级联反应的蛋白质）相互作用的伙伴。