An efficient one-pot synthetic protocol developed for the synthesis of pyrazolyl-2-thioxothiazolidin-4-one hybrid analogues by the condensation of pyrazole aldehydes, chloroacetic chloride and carbon disulfide using ammonium acetate. The synthesized compounds were screened for their in vitro antimicrobial activity against four bacterial and two fungal pathogens, the results showed that moderate to good zone of inhibition exhibited against all the tested organisms. The compounds structure was characterized based on spectral data such as ¹H-NMR, ¹³C-NMR, IR, mass spectrometry and elemental analysis.

中文翻译：

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一种高效一锅合成吡唑基-2-硫代噻唑并恶唑烷-4-酮杂种类似物并评估其抗菌活性

通过使用乙酸铵缩合吡唑醛，氯乙酸氯和二硫化碳，开发了一种有效的一锅合成方案，用于合成吡唑基-2-硫代噻唑并恶唑烷-4-一杂合类似物。筛选了合成的化合物对四种细菌和两种真菌病原体的体外抗菌活性，结果表明，对所有被测生物均显示出中等至良好的抑制区。基于光谱数据，例如¹ H-NMR，¹³ C-NMR，IR，质谱和元素分析来表征化合物的结构。