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Novel degradation pathways for Chlorpyrifos and 3, 5, 6-Trichloro-2-pyridinol degradation by bacterial strain Bacillus thuringiensis MB497 isolated from agricultural fields of Mianwali, Pakistan
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.pestbp.2020.104750 Samina Ambreen , Azra Yasmin
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.pestbp.2020.104750 Samina Ambreen , Azra Yasmin
Abstract Over use of organophosphate pesticides including Chlorpyrifos (CPF) has led to contamination of soil and water resources, resulting in serious health problems in humans along with other non-target organisms. The current study was aimed to investigate Chlorpyrifos as well as 3, 5, 6-Trichloro-2-pyridinol (TCP) biodegradation tendency of bacterial strain Bacillus thuringiensis MB497 isolated from wheat/cotton fields of Dera Saleemabad, Mianwali, Pakistan, having a history of heavy Organophosphate pesticides application. HPLC analysis revealed almost 99% degradation of the spiked CPF (200 mg L−1) in M-9 broth, soil slurry and soil microcosm by MB497 after 9 days of incubation. Strain MB497 was also able to degrade and transform TCP (28 mg L−1), up to 90.57% after 72 h of incubation in M-9 broth. A novel compound Di-isopropyl methanephosphonate along with known products of 3, 5, 6-Trichloro-2-pyridinol (TCP), Diethyl thiophospsphate and Phosphorothioic acid were detected as metabolites of CPF by GCMS analysis. Three novel metabolites of TCP (p-Propyl phenol, 2-Ethoxy-4, 4, 5, 5-tetramethyloxazoline and 3-(2, 4, 5-Trichlorophenoxy)-1-propyne) were identified after 72 h. Based on these metabolites, new/amended metabolic pathways for CPF and TCP degradation in these bacteria has been suggested.
中文翻译:
从巴基斯坦棉瓦里农田分离的苏云金芽孢杆菌 MB497 降解毒死蜱和 3, 5, 6-三氯-2-吡啶酚的新降解途径
摘要 包括毒死蜱 (CPF) 在内的有机磷农药的过度使用导致了土壤和水资源的污染,导致人类以及其他非目标生物的严重健康问题。本研究旨在探讨从巴基斯坦棉瓦里德拉萨勒马巴德麦/棉田中分离的苏云金芽孢杆菌 MB497 的毒死蜱和 3, 5, 6-Trichloro-2-pyridinol (TCP) 的生物降解趋势。重有机磷农药的应用。HPLC 分析显示,在孵育 9 天后,MB497 在 M-9 肉汤、土壤浆液和土壤微观世界中几乎 99% 降解了加标 CPF(200 mg L-1)。在 M-9 肉汤中培养 72 小时后,菌株 MB497 也能够降解和转化 TCP(28 mg L-1),高达 90.57%。通过 GCMS 分析检测到一种新型化合物二异丙基甲膦酸酯以及 3, 5, 6-三氯-2-吡啶醇 (TCP)、硫代磷酸二乙酯和硫代磷酸的已知产物作为 CPF 的代谢物。72 小时后鉴定出 TCP 的三种新型代谢物(p-Propyl phenol, 2-Ethoxy-4, 4, 5, 5-四甲基恶唑啉和 3-(2, 4, 5-Trichlorophenoxy)-1-propyne)。基于这些代谢物,提出了新的/修正的这些细菌中 CPF 和 TCP 降解的代谢途径。
更新日期:2021-02-01
中文翻译:
从巴基斯坦棉瓦里农田分离的苏云金芽孢杆菌 MB497 降解毒死蜱和 3, 5, 6-三氯-2-吡啶酚的新降解途径
摘要 包括毒死蜱 (CPF) 在内的有机磷农药的过度使用导致了土壤和水资源的污染,导致人类以及其他非目标生物的严重健康问题。本研究旨在探讨从巴基斯坦棉瓦里德拉萨勒马巴德麦/棉田中分离的苏云金芽孢杆菌 MB497 的毒死蜱和 3, 5, 6-Trichloro-2-pyridinol (TCP) 的生物降解趋势。重有机磷农药的应用。HPLC 分析显示,在孵育 9 天后,MB497 在 M-9 肉汤、土壤浆液和土壤微观世界中几乎 99% 降解了加标 CPF(200 mg L-1)。在 M-9 肉汤中培养 72 小时后,菌株 MB497 也能够降解和转化 TCP(28 mg L-1),高达 90.57%。通过 GCMS 分析检测到一种新型化合物二异丙基甲膦酸酯以及 3, 5, 6-三氯-2-吡啶醇 (TCP)、硫代磷酸二乙酯和硫代磷酸的已知产物作为 CPF 的代谢物。72 小时后鉴定出 TCP 的三种新型代谢物(p-Propyl phenol, 2-Ethoxy-4, 4, 5, 5-四甲基恶唑啉和 3-(2, 4, 5-Trichlorophenoxy)-1-propyne)。基于这些代谢物,提出了新的/修正的这些细菌中 CPF 和 TCP 降解的代谢途径。