Aim

The present study was designed to check the effect of daidzein in the management of diabetic retinopathy.

Main methods

Streptozotocin at dose 55 mg/kg was used for inducing diabetes in rats. After 28 days of diabetic induction, animals were treated with daidzein at dose 25, 50, and 100 mg/kg for the next 28 days. Electroretinography, estimation of plasma glucose, lactate dehydrogenase, aldose reductase, sorbitol dehydrogenase and oxidative stress parameters were performed at the end of the study. Histopathology of retina was carried out at the end of the study.

Key findings

Diabetic control animals showed a significant increase in levels of plasma glucose and plasma lactate dehydrogenase (p < 0.001). Treatment with daidzein at a dose of 50 and 100 mg/kg significantly reduced the elevated level of blood glucose (p < 0.01 and p < 0.01). Whereas, treatment with daidzein at a dose 100 mg/kg significantly reduced the elevated level of lactate dehydrogenase in plasma after 28 days of treatment (p < 0.01). Treatment with daidzein at a dose of 100 mg/kg significantly reduced the level of aldose reductase and sorbitol dehydrogenase (p < 0.01 and p < 0.001 respectively). Electroretinography revealed that daidzein treatment at a dose of 100 mg/kg significantly prevented the change in ‘a’ and ‘b’ wave amplitude and latency. Oxidative stress was also found to be significantly reduced after 28 days of daidzein treatment. Histopathological findings showed a reduction in retinal thickness after daidzein treatment.

Significance

Daidzein treatment protected retina from damage in hyperglycaemic conditions. Thus, Daidzein can be considered as an effective treatment option for diabetic retinopathy.

中文翻译：

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大豆苷元改善实验动物的糖尿病性视网膜病变

目标

本研究旨在检查大豆苷元在糖尿病性视网膜病治疗中的作用。

主要方法

剂量为55 mg / kg的链脲佐菌素用于诱导大鼠糖尿病。糖尿病诱导28天后，在接下来的28天中用大豆苷元以25、50和100 mg / kg的剂量治疗动物。在研究结束时进行了视网膜电图，血浆葡萄糖，乳酸脱氢酶，醛糖还原酶，山梨糖醇脱氢酶和氧化应激参数的估计。在研究结束时进行了视网膜的组织病理学检查。

主要发现

糖尿病对照组动物的血浆葡萄糖和血浆乳酸脱氢酶水平显着增加（p  <0.001）。用黄豆苷元50和100 mg / kg的剂量治疗可显着降低血糖水平升高（p  <0.01和p <0.01）。然而，在治疗28天后，以100 mg / kg的大豆黄酮剂量治疗可显着降低血浆中乳酸脱氢酶水平的升高（p  <0.01）。大豆黄酮100 mg / kg的剂量治疗可显着降低醛糖还原酶和山梨糖醇脱氢酶的水平（p  <0.01和p 分别<0.001）。视网膜电图显示，黄豆苷元剂量为100 mg / kg可以显着阻止'a'和'b'波振幅和潜伏期的改变。大豆苷元处理28天后，氧化应激也明显降低。组织病理学结果显示黄豆苷元治疗后视网膜厚度减少。

意义

黄豆苷元治疗可防止视网膜在高血糖情况下受损。因此，大豆黄酮可以被认为是糖尿病性视网膜病的有效治疗选择。