Plant protein sources play an essential role in aquaculture by reducing the use of fish meal to sustainable levels, although further supplementation is needed to fulfill fish nutritional requirements. This work addressed fish growth performance and proteome changes to dietary methionine in European seabass juveniles. A dose-dependent response to methionine (Met) was observed on fish growth consistent with proteomic analyses, suggesting Met requirement ≥0.9% (w/w). Fish fed at 0.77% (w/w) exhibited reduced growth and an enrichment in proteins involved in cellular homeostasis. Proteomics data suggest an optimal nutritional status at 1.36% Met (w/w), together with putative beneficial effects on the immune system up to 1.66% Met (w/w). The response to dietary Met involved the convergence of different metabolic and signalling pathways implicated in cell growth and immune response e.g., mTOR, Hedgehog or the T Cell receptor signalling, coupled with a fine-tuning regulation of amino acid metabolism and translation.

植物蛋白源通过将鱼粉的使用量减少到可持续的水平，在水产养殖中起着至关重要的作用，尽管需要进一步补充以满足鱼的营养需求。这项工作解决了欧洲鲈鱼幼鱼的鱼类生长性能和蛋白质蛋氨酸变化。在鱼的生长过程中，对蛋氨酸对蛋氨酸（Met）的剂量依赖性反应与蛋白质组学分析一致，表明Met需求≥0.9％（w / w）。以0.77％（w / w）喂食的鱼表现出生长减慢，并且参与细胞动态平衡的蛋白质富集。蛋白质组学数据表明最佳营养状态为蛋氨酸1.36％（w/ w），以及对免疫系统的高达1.66％Met（w / w）的推定有益作用。对饮食Met的反应涉及涉及细胞生长和免疫反应的不同代谢和信号途径的收敛，例如mTOR，Hedgehog或T细胞受体信号传导，以及氨基酸代谢和翻译的微调调节。