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Molecular screening for rickettsial bacteria and piroplasms in ixodid ticks surveyed from white-tailed deer (Odocoileus virginianus) and nilgai antelope (Boselaphus tragocamelus) in southern Texas
International Journal for Parasitology: Parasites and Wildlife ( IF 1.8 ) Pub Date : 2020-11-17 , DOI: 10.1016/j.ijppaw.2020.11.002
Pia U. Olafson , Beverly Greta Buckmeier , Melinda A. May , Donald B. Thomas

A survey of ixodid ticks parasitizing white-tailed deer (Odocoileus virginianus) and nilgai antelope (Boselaphus tragocamelus) was completed during the 2018–2019 public hunt season on the Laguna Atascosa National Wildlife Refuge (Cameron County, Texas) and the East Foundation's El Sauz Ranch in nearby Willacy County (Texas). Anocenter nitens was the predominant tick species identified with 5% of these ticks collected from nilgai. All life stages were encountered in high numbers on white-tailed deer, indicating that deer may be a primary host in this region. Amblyomma maculatum and Amblyomma inornatum were identified from both hosts, while Ixodes scapularis was encountered only on white-tailed deer. This is the first published record of A. inornatum on nilgai. A subset of ticks was used in PCR assays to detect Rickettsia spp., family Anaplasmataceae, Borrelia spp., and Theileria-Babesia spp. Borrelia spp. were not detected in any of the ticks analyzed. Rickettsia parkeri was detected in three A. maculatum adult ticks from deer, Rickettsia sp. endosymbiont sequences were present in all I. scapularis ticks, and Rickettsia amblyommatis was detected in three A. inornatum adult ticks from deer. Sequence analysis of Anaplasmataceae-positive amplicons from A. nitens and A. maculatum had low percent identity to published Anaplasma spp. sequences, suggesting a unique Anaplasma sp. may be circulating in the population. Anaplasma platys was detected from A. nitens larvae and an Ehrlichia sp. Delta strain was present in A. maculatum, both of unknown pathogenicity towards deer. Theileria cervi was detected in all stages of A. nitens ticks, and positive ticks originated from 27 of 31 deer and a single nilgai sampled from throughout the survey site. The primary vector for T. cervi is absent from this region, suggesting T. cervi is possibly maintained by a different tick species.



中文翻译:

从得克萨斯州南部的白尾鹿(Odocoileus virginianus)和尼尔加羚羊(Boselaphus tragocamelus)进行的ixodd s中立克次体细菌和质体的分子筛选

在2018-2019年拉古纳·阿塔斯科萨国家野生动物保护区(得克萨斯州卡梅伦县)的公共狩猎季节,完成了对寄生于白尾鹿(Odocoileus virginianus)和尼尔加羚羊(Boselaphus tragocamelus)的虱的调查。在附近的威拉西县(德克萨斯州)牧场。Anocenter nitens是主要的壁虱种类,其中有5%的ni是从nilgai收集的。在白尾鹿上遇到了所有生命阶段,这表明鹿可能是该地区的主要寄主。从两个宿主中都鉴定出了黄斑盲mbinornatum盲,,而肩x龙只在白尾鹿上遇到过。这是在尼尔基上首次公开的猪气单胞菌记录。PCR的一个子集用于PCR检测中,以检测立克次体无形科,疏螺旋体泰勒斯巴贝斯体疏螺旋体属。在任何分析的滴答声中均未检测到。在来自立克次体鹿的鹿的三个A. maculatum成年s中检测到立克次体parkeri。共生细菌序列是存在于所有的肩突硬蜱蜱,和立克次氏体amblyommatis在三个检测A. inornatum鹿成年s。序列分析Anaplasmataceae-从积极扩增A.鲷A. maculatum具有低的同一性百分比发表无形体属。序列,暗示独特的无浆菌。可能在人口中流传。无形体普拉提斯吉从检测A.鲷幼虫和埃立克体属。三角洲株存在于A. maculatum,既对鹿未知的致病性。在所有的A. nitens阶段都检测到了Theileria cervi31和正tick来自31头鹿中的27头,并且在整个调查地点都采样了一个ni。该区域不存在T.cervi的主要载体,这表明T. cervi可能由不同的壁虱物种维持。

更新日期:2020-11-21
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