This study investigated the treatment of 100-mg/L Norfloxacin (NOR) wastewater containing high concentrations of sulfate through a combination of electrolysis, sulfate-reducing up-flow sludge bed (SRUSB), and biological contact oxidation reactor (BCOR) treatments. Results revealed that after 62 h, the reaction system had processed over 97% of the NOR. Additionally, electrolysis with sodium sulfate as the electrolyte transformed 87.8% of the NOR but only 33.5% of the total organic carbon (TOC). In the SRUSB, the TOC and SO₄²⁻ contents were simultaneously reduced by 87.4% and 95.6%, respectively, providing a stable environment to the BCOR. In the BCOR, 36.3% and 85.9% of the NOR and TOC were degraded. High-performance liquid chromatography-tandem mass spectrometry analysis identified three possible degradation pathways under the attack of –OH during electrolysis, including defluorination, piperazinyl ring transformation, and quinolone ring transformation. Furthermore, the Illumina HiSeq sequencing results demonstrated that the sulfate-reducing bacteria (represented by Desulfobacter and Desulfobulbus) in the SRUSB and the sulfate-oxidizing bacteria (mainly consisting of Gammaproteobacteria and Alphaproteobacteria) in the BCOR played important roles in carbon chain oxidation and benzene ring opening and thoroughly degraded the electrolysis products. Thus, this method effectively overcomes the incomplete degradation and low removal efficiency issues associated with single electrolysis or biological methods in traditional processes.

这项研究研究了通过电解，减少硫酸盐的上流污泥床（SRUSB）和生物接触氧化反应器（BCOR）的组合处理100 mg / L含高浓度硫酸盐的诺氟沙星（NOR）废水的方法。结果显示，在62小时后，反应系统处理了NOR的97％以上。另外，用硫酸钠作为电解质进行的电解转化了NOR的87.8％，但仅转化了总有机碳（TOC）的33.5％。在SRUSB中，TOC和SO ₄ ²⁻同时减少了87.4％和95.6％的含量，为BCOR提供了稳定的环境。在BCOR中，NOR和TOC的降解率为36.3％和85.9％。高效液相色谱-串联质谱分析确定了电解过程中-OH攻击下三种可能的降解途径，包括脱氟，哌嗪基环转化和喹诺酮环转化。此外，Illumina HiSeq测序结果表明，SRUSB中的硫酸盐还原细菌（以Desulfobacter和Desulfobulbus代表）和硫酸盐氧化细菌（主要由γ-变形杆菌和α-变形杆菌组成））中的BCOR在碳链氧化和苯环打开以及彻底降解电解产物方面起着重要作用。因此，该方法有效地克服了传统工艺中与单一电解或生物方法相关的不完全降解和去除效率低的问题。