Chlorothalonil (CLT) is a broad spectrum, and non-systemic fungicide applied in foliar structures to prevent and treat pathogens. This compound reaches to aquatic environments and affects the biota. In this context, the main goal of this study was to assess the effects of CLT at biochemical, tissular, and individual levels of biological organization using the invasive bivalve Corbicula largillierti as a bioindicator species. Clams were exposed to different sublethal concentrations (0, 10, 20 and 50 µg. L⁻¹ CLT) for 96 h. At biochemical level, the enzymatic activity (Glutathione-s-Transferase, Catalase, Acetyl-, Butiryl- and Carboxyl-esterases) and lipid peroxidation were measured in gills and the visceral mass. Also, the digestive gland morphometry through quantitative histological indexes was registered at the tissular level. Finally, filtering activity and burial behavior at the individual level were measured. At the highest CLT concentration, the most significant changes were observed in enzymatic activity (except for butyrylcholinesterase), lipid peroxidation and in digestive gland morphometry. It was also registered increases of the filtering activity and the latency time to burial. Most of the biomarkers assessed showed significant responses under CLT exposure. Therefore, taking into account that C. largillierti was affected by CLT, it can be expected that other species could be in a potential risk if this fungicide is present in freshwater systems.

氯噻酮（CLT）是一种广谱的非系统性杀菌剂，应用于叶面结构中，以预防和治疗病原体。该化合物可到达水生环境并影响生物区系。在这种情况下，本研究的主要目标是使用侵入性双壳双瓣锦葵科作为生物指示剂物种，评估CLT对生物化学，组织和个体生物组织水平的影响。蛤暴露于不同的亚致死浓度（0、10、20和50 µg。L ^-1CLT）96小时。在生化水平上，测量g和内脏质量的酶活性（谷胱甘肽-s-转移酶，过氧化氢酶，乙酰基，丁酰和羧基酯酶）和脂质过氧化。另外，通过定量组织学指标进行的消化腺形态测定在组织水平上进行了记录。最后，在个体水平上测量过滤活性和埋葬行为。在最高的CLT浓度下，在酶活性（丁酰胆碱酯酶除外），脂质过氧化作用和消化腺形态学方面观察到最显着的变化。它也记录了过滤活动和埋葬潜伏时间的增加。评估的大多数生物标志物在CLT暴露下均显示出明显的反应。因此，考虑到C. largillierti受到了CLT的影响，如果淡水系统中存在这种杀菌剂，则可以预计其他物种可能会面临潜在风险。