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Phosphate availability and ectomycorrhizal symbiosis with Pinus sylvestris have independent effects on the Paxillus involutus transcriptome
Mycorrhiza ( IF 3.9 ) Pub Date : 2020-11-16 , DOI: 10.1007/s00572-020-01001-6
Christina Paparokidou , Jonathan R. Leake , David J. Beerling , Stephen A. Rolfe

Many plant species form symbioses with ectomycorrhizal fungi, which help them forage for limiting nutrients in the soil such as inorganic phosphate (Pi). The transcriptional responses to symbiosis and nutrient-limiting conditions in ectomycorrhizal fungal hyphae, however, are largely unknown. An artificial system was developed to study ectomycorrhizal basidiomycete Paxillus involutus growth in symbiosis with its host tree Pinus sylvestris at different Pi concentrations. RNA-seq analysis was performed on P. involutus hyphae growing under Pi-limiting conditions, either in symbiosis or alone. We show that Pi starvation and ectomycorrhizal symbiosis have an independent effect on the P. involutus transcriptome. Notably, low Pi availability induces expression of newly identified putative high-affinity Pi transporter genes, while reducing the expression of putative organic acid transporters. Additionally, low Pi availability induces a close transcriptional interplay between P and N metabolism. GTP-related signalling was found to have a positive effect in the maintenance of ectomycorrhizal symbiosis, whereas multiple putative cytochrome P450 genes were found to be downregulated, unlike arbuscular mycorrhizal fungi. We provide the first evidence of global transcriptional changes induced by low Pi availability and ectomycorrhizal symbiosis in the hyphae of P. involutus, revealing both similarities and differences with better-characterized arbuscular mycorrhizal fungi.



中文翻译:

樟子松的磷酸盐有效性和根外共生对Paxillus involutus转录组有独立影响

许多植物物种与外生菌根真菌形成共生体,这有助于它们觅食以限制土壤中的营养物,例如无机磷酸盐(Pi)。然而,外生菌根真菌菌丝中对共生和营养限制条件的转录反应尚不清楚。开发了一个人工系统来研究在不同Pi浓度下,其宿主树樟子松共生共生的外生菌根茎线虫Paxillus involutus生长。在共生或单独存在的条件下,对在Pi限制条件下生长的渐近线疟原虫菌丝进行RNA-seq分析。我们表明,Pi饥饿和外生菌根共生对P. involutus有独立的影响转录组。值得注意的是,低的Pi利用率会诱导新鉴定的假定的高亲和力Pi转运蛋白基因的表达,同时会降低假定的有机酸转运蛋白的表达。此外,低的Pi利用率会引起P和N代谢之间的紧密转录相互作用。与丛枝菌根真菌不同,发现与GTP相关的信号传导在维持外生菌根共生中具有积极作用,而发现多个推定的细胞色素P450基因被下调。我们提供了由低磷利用率和外生菌根菌丝菌丝中的菌根共生引起的全球转录变化的第一个证据,揭示了与特征更好的丛枝菌根真菌的相似性和差异性。

更新日期:2020-11-17
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