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Development of microsatellite markers for the predatory mite Phytoseiulus macropilis and cross-amplification in three other species of phytoseiid mites
Experimental and Applied Acarology ( IF 2.2 ) Pub Date : 2020-11-16 , DOI: 10.1007/s10493-020-00572-5
Maria Cristina Vitelli Queiroz , Fernanda Ancelmo de Oliveira , Anete Pereira de Souza , Mario Eidi Sato

Phytoseiid mites are efficient predators of mites and small pest insects. Understanding the dispersion and distribution pattern of phytoseiid mites is essential to promote the conservation of these natural enemies and support their use in biological control. Population genetic studies using molecular markers such as microsatellites have proved to be extremely informative to address questions about population structure and dispersion patterns of predatory mites. The objective of this work was to develop specific microsatellite markers for the predatory mite Phytoseiulus macropilis, aiming at improving field dispersion studies. For this purpose, the genomic DNA was extracted from the whole body of a pool of 260 adult females and used to build the genomic microsatellites-enriched library, using biotinylated probes (CT)8 and (GT)8. In total 26 pairs of primers were synthesized and screened across 30 adult females of P. macropilis for characterization. Seven loci were polymorphic, revealing from two to six alleles per locus. Cross amplifications were successfully obtained in the species Phytoseiulus persimilis, Amblyseius swirskii and Proprioseiopsis sp. The molecular markers obtained are the first developed for P. macropilis—they are effective for the detection and quantification of genetic variation, and show high transferability, thus can be used in genetic and molecular studies of this and other species of the same genus and also of close genera.



中文翻译:

开发捕食性螨Phytoseiulus macropilis的微卫星标记,并在其他三种植物性螨中进行交叉扩增

拟南芥螨是螨和小型害虫的有效捕食者。了解植物寄生螨的扩散和分布模式对于促进这些天敌的保护和支持其在生物防治中的使用至关重要。事实证明,使用分子标记(例如微卫星)进行的遗传研究对于解决有关捕食性螨的种群结构和分布模式的问题非常有用。这项工作的目的是为捕食性螨Phytoseiulus macropilis开发特定的微卫星标记,旨在改善场扩散研究。为此,从260名成年雌性的整个池中提取基因组DNA,并使用生物素化探针(CT)8和(GT)8将其用于构建富含基因组微卫星的文库。总共合成了26对引物,并在30名成年雌性大果假单胞菌中进行了筛选,以进行鉴定。七个基因座是多态的,每个位点揭示了两个到六个等位基因。交叉扩增已成功地获得了Phytoseiulus persimilisAmblyseius swirskiiProprioseiopsis sp。种。所获得的分子标记物是最先为大型假单胞菌开发的-它们可有效地检测和定量遗传变异,并显示出高度的可转移性,因此可用于该属和其他属以及近亲属的遗传和分子研究。

更新日期:2020-11-17
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