Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were simulated, and the turbulence, dissolved oxygen and shear strain rate of the bioreactor were analyzed. The results showed that the quasi-steady-state flow patterns of the unbaffled shaking bioreactors were broken for the bioreactors with the strengthening effects of baffles. The mixing and the oxygen volumetric mass transfer coefficient (k_La) (simulated results) were improved significantly, and the shear strain rates were also increased greatly for the baffle bioreactors. The shear strain rates of the baffle bioreactors were mainly in the range of 0–20 s⁻¹, and they were still low enough for CHO cell cultures.

一次性定轨摇动生物反应器已广泛用于哺乳动物细胞悬浮培养。本工作设计了三种挡板结构：垂直挡板、斜挡板和水平挡板。模拟了具有不同挡板结构的振动生物反应器的流场，分析了生物反应器的湍流、溶解氧和剪切应变率。结果表明，具有挡板强化作用的生物反应器打破了无挡板振动生物反应器的准稳态流动模式。混合和氧气体积传质系数 ( k _L a)（模拟结果）得到显着改善，并且挡板生物反应器的剪切应变率也大大提高。挡板生物反应器的剪切应变率主要在 0-20 s ^-1的范围内，它们对于 CHO 细胞培养仍然足够低。