Nerve guidance conduits (NGCs) composed of biocompatible polymers have been attracting attention as an alternative for autograft surgery in peripheral nerve regeneration. However, the nerve tissues repaired by NGCs often tend to be inadequate and lead to functional failure because of the lack of cellular supports. This paper presents a chitosan-collagen hydrogel conduit containing cells to induce peripheral nerve regeneration with cellular support. The conduit composed of two coaxial hydrogel layers of chitosan and collagen is simply made by molding and mechanical anchoring attachment with holes made on the hydrogel tube. A chitosan layer strengthens the conduit mechanically, and a collagen layer provides a scaffold for cells supporting the axonal extension. The conduits of different diameters (outer diameter approximately 2–4 mm) are fabricated. The conduit is bioabsorbable with lysozyme, and biocompatible even under bio absorption. In the neuron culture demonstration, the conduit containing Schwann cells induced the extension of the axon of neurons directed to the conduit. Our easily fabricated conduit could help the high-quality regeneration of peripheral nerves and contribute to the nerve repair surgery.

由生物相容性聚合物组成的神经引导导管 (NGC) 作为外周神经再生中自体移植手术的替代方案一直备受关注。然而，NGCs修复的神经组织往往不足，由于缺乏细胞支持而导致功能衰竭。本文介绍了一种含有细胞的壳聚糖 - 胶原水凝胶导管，可在细胞支持下诱导周围神经再生。由壳聚糖和胶原蛋白的两个同轴水凝胶层组成的导管简单地通过模制和机械锚定附件制成，并在水凝胶管上打孔。壳聚糖层以机械方式加强导管，胶原层为支持轴突延伸的细胞提供支架。制造了不同直径（外径约 2-4 毫米）的导管。该导管可通过溶菌酶进行生物吸收，并且即使在生物吸收下也具有生物相容性。在神经元培养演示中，含有雪旺氏细胞的导管诱导神经元轴突延伸至导管。我们易于制造的导管可以帮助周围神经的高质量再生，并有助于神经修复手术。