The number of people at risk for dengue viral infection is about 50% of the worlds population. Dengue viral infection is classified as dengue fever and severe dengue depending on the severity. Severe dengue may be associated with infection by the different serotype from the primary infected serotype. The circulating epidemic serotypes have changed continuously and periodically. It is very important to diagnose dengue viral infection as early as possible to be treated adequately. The purpose of this study was the development of a simultaneous detection method for all the serotypes of dengue virus in a single sample. The consensus primers and four different fluorescein-tagged probes that specifically binds to each serotype of dengue virus were designed from the multiple alignment data of full-length dengue viral sequences, which were retrieved from GenBank. They were located between the 5’ untranslated region and the capsid gene of the dengue viruses. Each serotype of dengue viruses isolated in Korea was detected correctly with the established multiplex real time RT-PCR at the level of 5-10 copies/reaction. Other RNA viruses such as the Japanese encephalitis virus, Zika virus, yellow fever virus, and Chikungunya virus were not amplified with this method. The results of the inter-assays and intra-assays were within the acceptable variation ranges. Conclusively, the developed method can detect each serotype of dengue virus specifically and quantitatively even when different serotypes of dengue virus are present in a sample.

有登革热病毒感染风险的人数约占世界人口的50％。登革热病毒感染根据严重程度分为登革热和严重登革热。严重的登革热可能与原发感染血清型不同而导致感染。流行的流行血清型已经连续不断地变化。尽早诊断出登革热病毒感染并加以适当治疗非常重要。这项研究的目的是开发一种同时检测单个样本中所有登革热病毒血清型的方法。根据全长登革热病毒序列的多重比对数据，设计了共有引物和四种不同的荧光素标记探针，它们分别与登革热病毒的每种血清型结合。从GenBank检索到的。它们位于5'非翻译区和登革热病毒的衣壳基因之间。在韩国分离的登革热病毒的每种血清型都可以通过已建立的多重实时RT-PCR以5-10拷贝/反应的水平正确检测。该方法未扩增其他RNA病毒，例如日本脑炎病毒，寨卡病毒，黄热病病毒和基孔肯雅病毒。批间和批内的结果均在可接受的变异范围内。最终，即使样品中存在不同血清型的登革热病毒，也可以特异性，定量地检测出每种血清型的登革热病毒。在韩国分离的登革热病毒的每种血清型都可以通过已建立的多重实时RT-PCR以5-10拷贝/反应的水平正确检测。该方法未扩增其他RNA病毒，例如日本脑炎病毒，寨卡病毒，黄热病病毒和基孔肯雅病毒。批间和批内的结果均在可接受的变异范围内。最终，即使样品中存在不同血清型的登革热病毒，也可以特异性，定量地检测出每种血清型的登革热病毒。在韩国分离的登革热病毒的每种血清型都可以通过已建立的多重实时RT-PCR以5-10拷贝/反应的水平正确检测。该方法未扩增其他RNA病毒，例如日本脑炎病毒，寨卡病毒，黄热病病毒和基孔肯雅病毒。批间和批内的结果均在可接受的变异范围内。最终，即使样品中存在不同血清型的登革热病毒，也可以特异性，定量地检测出每种血清型的登革热病毒。批间和批内的结果均在可接受的变异范围内。最终，即使样品中存在不同血清型的登革热病毒，也可以特异性，定量地检测出每种血清型的登革热病毒。批间和批内的结果均在可接受的变异范围内。最终，即使样品中存在不同血清型的登革热病毒，也可以特异性，定量地检测出每种血清型的登革热病毒。