Poa L. is a large genus of grass in Gramineae, among which P. pratensis is widely cultivated as turf and forage. Satellite DNA is the main components of the plant genome. Information of satellites will helpful for dissection the genome composition and definition of the phylogeny relationship of these species. However, the knowledge about the satellites in genus Poa is still limited. Four satellite DNAs were identified using the Repeat Explorer pipeline in HiSeq Illumina reads from diploid plants in P. malaca (2n = 26). Two satellites showed high similarity with the previously identified PpTr-1 and PpTr-3, whereas two others are newly identified with the monomer of 326 bp (Poa-326) and 353 bp (Poa-353) respectively. The clone DNAs of PpTr-1 and PpTr-3, and oligonucleotides designed representing satellites Poa-326 and Poa-353 were probed to test on chromosomes across 13 Poa speceis with different polyploidy level by fluorescent in situ hybridization (FISH). PpTr-1, PpTr-3, and Poa-362 were stably positioned in the subtelomeric regions in nearly all species with the variation of hybridization sites number. However, Poa-353 showed different FISH patterns of multiple regions with the variation of hybridization intensity and distribution sites across species. In addition, 5S rDNA and 45S rDNA were used to characterize the genome of the Poa species. Four rDNA FISH patterns were revealed in the tested species. Four identified satellite were high conservable across Poa species. Genome distribution of these satellites can be characterized by FISH. The variation of satellite DNAs and rDNA chromosomal distributions between species provide useful information for phylogenetic analysis in genus Poa.

中文翻译：

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Poa L中卫星DNA的鉴定和表征

禾本科（Poa L.）是禾本科中的一种大型草，其中普拉提草被广泛用作草皮和牧草。卫星DNA是植物基因组的主要成分。卫星信息将有助于解剖这些物种的基因组组成和系统发育关系。但是，关于Poa属中的卫星的知识仍然有限。使用HiSeq Illumina的Repeat Explorer管道从疟疾假单胞菌（2n = 26）的二倍体植物中读取了四个卫星DNA。两颗卫星与先前鉴定的PpTr-1和PpTr-3具有高度相似性，而另两颗卫星则分别被分别鉴定为326 bp（Poa-326）和353 bp（Poa-353）的单体。PpTr-1和PpTr-3的克隆DNA，并设计了代表卫星Poa-326和Poa-353的寡核苷酸，通过荧光原位杂交（FISH）检测了13种不同多倍体水平的Poa物种的染色体。随着杂交位点数目的变化，PpTr-1，PpTr-3和Poa-362稳定地定位在几乎所有物种的亚端粒区域中。然而，Poa-353在多个区域显示出不同的FISH模式，其中杂交强度和物种间分布位点的变化。此外，使用5S rDNA和45S rDNA表征Poa物种的基因组。在被测物种中发现了四个rDNA FISH模式。在Poa物种中，四颗已确认的卫星是高度保守的。这些卫星的基因组分布可以用FISH来表征。