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Functional identification of PsMYB57 involved in anthocyanin regulation of tree peony
BMC Genetics ( IF 2.9 ) Pub Date : 2020-11-16 , DOI: 10.1186/s12863-020-00930-7
Yanzhao Zhang , Shuzhen Xu , Yanwei Cheng , Jing Wang , Xiangxiang Wang , Runxiao Liu , Jianming Han

R2R3 myeloblastosis (MYB) genes are widely distributed in plants and comprise one of the largest transcription factor gene families. They play important roles in the regulatory networks controlling development, metabolism, and stress responses. Researches on functional genes in tree peony are still in its infancy. To date, few MYB genes have thus far been reported. In this study, we constructed a comprehensive reference gene set by transcriptome sequencing to obtain R2R3 MYB genes. The transcriptomes of eight different tissues were sequenced, and 92,837 unigenes were obtained with an N50 of 1662 nt. A total of 48,435 unigenes (77.98%) were functionally annotated in public databases. Based on the assembly, we identified 57 R2R3 MYB genes containing full-length open reading frames, which clustered into 35 clades by phylogenetic analysis. PsMYB57 clustered with anthocyanin regulation genes in Arabidopsis and was mainly transcribed in the buds and young leaves. The overexpression of PsMYB57 induced anthocyanin accumulation in tobacco, and four detected anthocyanin structural genes, including NtCHS, NtF3’H, NtDFR, and NtANS, were upregulated. The two endogenous bHLH genes NtAn1a and NtAn1b were also upregulated and may work in combination with PsMYB57 in regulating anthocyanin structural genes. Our study offers a useful reference to the selection of candidate MYB genes for further functional studies in tree peony. Function analysis of PsMYB57 is helpful to understand the color accumulation in vegetative organs of tree peony. PsMYB57 is also a promising resource to improve plant color in molecular breeding.

中文翻译:

PsMYB57参与牡丹花色苷调节的功能鉴定

R2R3骨髓成纤维细胞病(MYB)基因广泛分布在植物中,并且是最大的转录因子基因家族之一。它们在控制发育,新陈代谢和应激反应的调控网络中发挥重要作用。对牡丹功能基因的研究仍处于起步阶段。迄今为止,迄今尚未报道过MYB基因。在这项研究中,我们通过转录组测序构建了一个全面的参考基因集,以获得R2R3 MYB基因。测序了八个不同组织的转录组,并获得了92,837个单基因,N50为1662 nt。公共数据库中共有48,435个单基因(77.98%)在功能上进行了注释。基于该大会,我们确定了包含全长开放阅读框的57个R2R3 MYB基因,通过系统发育分析将其聚集成35个进化枝。PsMYB57在拟南芥中聚集着花色苷调节基因,主要转录在芽和幼叶中。PsMYB57的过度表达诱导了烟草中花色苷的积累,并且四个检测到的花色苷结构基因,包括NtCHS,NtF3'H,NtDFR和NtANS被上调。两个内源性bHLH基因NtAn1a和NtAn1b也被上调,可能与PsMYB57结合使用以调节花色苷结构基因。我们的研究为选择候选MYB基因提供了有用的参考,以进一步进行牡丹的功能研究。PsMYB57的功能分析有助于了解牡丹营养器官中的颜色积累。PsMYB57还是在分子育种中改善植物颜色的有前途的资源。
更新日期:2020-11-16
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