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The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-11-16 , DOI: 10.2147/ijn.s268657
Haiyan Wang 1 , Qiuping Xu 1 , Hui Hu 2 , Chunling Shi 3 , Ziyan Lin 3 , Huixi Jiang 3 , Huaipu Dong 3 , Jing Guo 1
Affiliation  

Background: Relying on surface topography alone to enhance the osteointegration of implants is still inadequate. An effective way to combine long-term ion release and surface topography to enhance osteogenic property is urgently needed.
Purpose: The objective of this study is to fabricate a long-term strontium ion release implant system and confirm the biological function in vitro and in vivo.
Methods: The biomimic surface was fabricated through alkali-heat treatment and magnetron sputtering. The in vitro biological function assays were determined by MTT, fluorescence staining, alkaline phosphatase activity, extracellular mineralization, and quantitative real-time polymerase chain reaction assays. The in vivo experiments were detected by micro-CT, HE staining and Masson staining.
Results: The biomimic surface structure has been successfully fabricated. The in vitro cell assays determined that AH-Ti/Sr90 possessed the best biological function. The in vivo experiments demonstrated that AH-Ti/Sr90 could promote osteointegration significantly under both in normal and osteoporotic conditions.
Conclusion: We determined that AH-Ti/Sr90 possesses the best osteogenic property, long-term ion release capacity and osteointegration promotion ability. It has potential clinic application prospects.



中文翻译:

锶改性分级微纳米钛植入物的制备及作用

背景:仅依靠表面形貌来增强种植体的骨整合仍然是不够的。迫切需要一种将长期离子释放和表面形貌相结合以增强成骨特性的有效方法。
目的:本研究的目的是制造一种长效锶离子释放植入系统,并在体外和体内确认其生物学功能。
方法:通过碱热处理和磁控溅射制备仿生表面。通过MTT、荧光染色、碱性磷酸酶活性、细胞外矿化和定量实时聚合酶链反应测定确定体外生物功能测定。体内实验通过显微CT、HE染色和Masson染色进行检测。
结果:成功制备了仿生表面结构。体外细胞试验确定AH-Ti/Sr90具有最好的生物学功能。体内实验表明,AH-Ti/Sr90 在正常和骨质疏松条件下均能显着促进骨整合。
结论:我们确定AH-Ti/Sr90具有最佳的成骨性能、长期离子释放能力和骨整合促进能力。具有潜在的临床应用前景。

更新日期:2020-11-16
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