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Modulation of Neuronal Cell Affinity on PEDOT–PSS Nonwoven Silk Scaffolds for Neural Tissue Engineering
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2020-11-16 , DOI: 10.1021/acsbiomaterials.0c01239
Adrián Magaz 1, 2 , Ben F. Spencer 1 , John G. Hardy 3, 4 , Xu Li 2, 5 , Julie E. Gough 1 , Jonny J. Blaker 1, 6
Affiliation  

Peripheral nerve injury is a common consequence of trauma with low regenerative potential. Electroconductive scaffolds can provide appropriate cell growth microenvironments and synergistic cell guidance cues for nerve tissue engineering. In the present study, electrically conductive scaffolds were prepared by conjugating poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT–PSS) or dimethyl sulfoxide (DMSO)-treated PEDOT–PSS on electrospun silk scaffolds. Conductance could be tuned by the coating concentration and was further boosted by DMSO treatment. Analogue NG108-15 neuronal cells were cultured on the scaffolds to evaluate neuronal cell growth, proliferation, and differentiation. Cellular viability was maintained on all scaffold groups while showing comparatively better metabolic activity and proliferation than neat silk. DMSO-treated PEDOT–PSS functionalized scaffolds partially outperformed their PEDOT–PSS counterparts. Differentiation assessments suggested that these PEDOT–PSS assembled silk scaffolds could support neurite sprouting, indicating that they show promise to be used as a future platform to restore electrochemical coupling at the site of injury and preserve normal nerve function.

中文翻译:

用于神经组织工程的PEDOT-PSS无纺丝支架上神经细胞亲和力的调节

周围神经损伤是具有低再生潜力的创伤的常见后果。导电支架可以为神经组织工程提供适当的细胞生长微环境和协同细胞指导线索。在本研究中,通过将电纺丝支架上的聚(3,4-乙撑二氧噻吩)-聚苯乙烯磺酸盐(PEDOT-PSS)或二甲亚砜(DMSO)处理的PEDOT-PSS共轭来制备导电支架。可以通过涂层浓度来调节电导率,并且通过DMSO处理可以进一步提高电导率。在支架上培养类似物NG108-15神经元细胞,以评估神经元细胞的生长,增殖和分化。在所有支架组上维持细胞活力,同时显示出比纯丝更好的代谢活性和增殖。经DMSO处理的PEDOT-PSS功能化支架部分优于其PEDOT-PSS支架。差异评估表明,这些PEDOT-PSS组装的丝绸支架可以支持神经突萌发,表明它们显示出有望用作将来在损伤部位恢复电化学耦合并保持正常神经功能的平台。
更新日期:2020-12-14
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