For connecting flow‐through analytical methods with capillary electrophoresis, a chip working in the air‐assisted flow gating interface regime is cast from poly(dimethylsiloxane). In the injection space, the exit from the delivery capillary is placed close to the entrance to the separation capillary. Prior to injecting the sample into the separation capillary, the background electrolyte is forced out of the injection space by a stream of air. In the empty space, a drop of the sample with a volume of <100 nL is formed between the exit from the delivery capillary and the entrance into the separation capillary, from which the sample is injected hydrodynamically into the separation capillary. After injection, the injection space is filled with BGE, and the separation can be begun. Three geometric variants for the mutual geometric arrangement of the delivery and separation capillaries were tested: the delivery capillary is placed perpendicular to the separation capillary, from either above or below, or the capillaries are placed axially, that is, directly opposite one another. All of the variants are equivalent from the analytical and separation efficiency viewpoints. The repeatability expressed by RSD is up to 5%. The tested flow gating interface variants are also suitable for continuous and discontinuous sampling at flow rates of the order of units of μL/min. The developed instrument for sequential electrophoretic analysis operates fully automatically and is suitable for rapid sequential monitoring of dynamic processes.

中文翻译：

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毛细管电泳“空气辅助”流动门控界面的各种几何排列的表征

为了将流通分析方法与毛细管电泳连接起来，在空气辅助流动门控界面机制下工作的芯片由聚（二甲基硅氧烷）浇铸而成。在进样空间中，输送毛细管的出口靠近分离毛细管的入口。在将样品注入分离毛细管之前，背景电解液被一股气流压出注入空间。在空的空间中，在输送毛细管的出口和分离毛细管的入口之间形成一滴体积小于 100 nL 的样品，样品通过流体动力学注入分离毛细管。注入后，注入空间充满BGE，即可开始分离。测试了输送和分离毛细管相互几何排列的三种几何变体：输送毛细管垂直于分离毛细管从上方或下方放置，或者毛细管轴向放置，即彼此直接相对。从分析和分离效率的角度来看，所有变体都是等效的。以 RSD 表示的重复性高达 5%。经测试的流动门控接口变体也适用于以 μL/min 为单位的流速的连续和不连续采样。开发的顺序电泳分析仪全自动运行，适用于动态过程的快速顺序监测。输送毛细管垂直于分离毛细管从上方或下方放置，或者毛细管轴向放置，即彼此直接相对。从分析和分离效率的角度来看，所有变体都是等效的。以 RSD 表示的重复性高达 5%。经测试的流动门控接口变体也适用于以 μL/min 为单位的流速的连续和不连续采样。开发的顺序电泳分析仪全自动运行，适用于动态过程的快速顺序监测。输送毛细管垂直于分离毛细管从上方或下方放置，或者毛细管轴向放置，即彼此直接相对。从分析和分离效率的角度来看，所有变体都是等效的。以 RSD 表示的重复性高达 5%。经测试的流动门控接口变体也适用于以 μL/min 为单位的流速的连续和不连续采样。开发的顺序电泳分析仪全自动运行，适用于动态过程的快速顺序监测。以 RSD 表示的重复性高达 5%。经测试的流动门控接口变体也适用于以 μL/min 为单位的流速的连续和不连续采样。开发的顺序电泳分析仪全自动运行，适用于动态过程的快速顺序监测。以 RSD 表示的重复性高达 5%。经测试的流动门控接口变体也适用于以 μL/min 为单位的流速的连续和不连续采样。开发的顺序电泳分析仪全自动运行，适用于动态过程的快速顺序监测。