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Genome-wide association mapping for key seed metabolites using a large panel of natural and derived forms of Brassica rapa L.
Industrial Crops and Products ( IF 5.9 ) Pub Date : 2020-11-16 , DOI: 10.1016/j.indcrop.2020.113073
Snehdeep Kaur , Javed Akhatar , Harjeevan Kaur , Chhaya Atri , Meenakshi Mittal , Anna Goyal , Usha Pant , Gurpreet Kaur , Surinder S. Banga

Brassica rapa L. (AA; 2n = 20) store considerable amounts of oil and protein reserves in the seeds. Genetic enhancement of these seed storage products is sought to meet specific requirements of the producers and consumers of edible oils, biofuels, animal feed and other industrial products. This necessitates the introduction of new genetic variation and a greater understanding of the trait genetics. We tested 195 germplasm lines of B. rapa for oil, protein and glucosinolates under the environment conditions differing for day length and ambient temperatures during development and maturation of the seeds. This helped to document phenotypic variations for these traits and also identify accessions with extreme phenotypes. DArT genotyping and genome wide association studies enabled detection of SNP markers within or near the genes that are associated directly or indirectly with the biosynthesis of oil (LTPG5, FRK7, SSE1, LACS8, LTPG33), protein (VTI1B, TSB2, SESA4, bZIP1, PPI1, K18J17.13) and glucosinolates (IMD1, MYB119, ILR3, IQD1, ADS2, SOT18). We also envisaged a gene, PLAT2. It is a member of PLAT-plant-stress subgroup of proteins. PLAT2 can be a target for breeding efforts aimed at improving abiotic stress tolerance. Introduction of novel allelic diversity may be more desirable for the development of designer genotypes rather than the simple repackaging of the genetic variation extant in the crop gene pools. It is for the first time that an attempt has been made to characterize allelic diversity extant in the ‘A’ sub genome of B. juncea for its deployment in B. rapa genepool.



中文翻译:

全基因组关联映射的关键种子代谢物使用大量的天然和衍生形式的甘蓝型油菜

甘蓝型油菜(AA; 2n = 20)在种子中储存了大量的油和蛋白质。寻求这些种子存储产品的遗传增强以满足食用油,生物燃料,动物饲料和其他工业产品的生产者和消费者的特定要求。这就需要引入新的遗传变异并加深对性状遗传的了解。我们测试了B. rapa的195种质系在种子发育和成熟期间因天长和环境温度不同而不同的环境条件下油,蛋白质和芥子油苷的含量。这有助于记录这些性状的表型变异,也有助于鉴定具有极端表型的材料。DArT基因分型和全基因组关联研究使人们能够检测与油(LTPG5FRK7SSE1LACS8LTPG33),蛋白质(VTI1BTSB2SESA4bZIP1PPI1K18J17.13)和芥子油苷(IMD1MYB119ILR3IQD1ADS2SOT18)。我们还设想了一个基因PLAT2。它是PLAT植物胁迫蛋白亚组的成员。PLAT2可以成为旨在改善非生物胁迫耐受性的育种目标。引入新的等位基因多样性可能更适合于设计基因型的开发,而不是简单地重新包装作物基因库中现有的遗传变异。这是第一次尝试表征芥菜油菜'A'亚基因组中现存的等位基因多样性,以便将其部署在油菜 基因库。

更新日期:2020-11-16
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