This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and containing carbon nanotubes, for immobilization of Paracoccus yeei VKM B-3302 bacteria. The structures of produced polymers were studied by IR spectroscopy and scanning electron microscopy. Cyclic voltammetry and impedance spectroscopy found the electrochemical characteristics of the investigated systems: the heterogeneous electron transfer rate constant, the constant of the rate of interaction with P. yeei bacteria and the impedance. The systems containing carbon nanotubes and ferrocene-based redox-active polymer proved to be the most promising. Biosensors formed using the hybrid polymers had a high sensitivity with the lower boundary of 0.1 mg/dm3 of the detected BOD5 concentrations and a high correlation (R = 0.9916) with the standard BOD assay of surface water samples.

中文翻译：

---

使用基于碳纳米管和改性有机基质的生物相容性氧化还原活性聚合物开发高灵敏度 BOD 生物传感器

这项工作研究了基于牛血清白蛋白和壳聚糖的氧化还原活性聚合物的使用，该聚合物与中性红和二茂铁的介质共价结合并含有碳纳米管，用于固定 Paracoccus yeei VKM B-3302 细菌。通过红外光谱和扫描电子显微镜研究了制备的聚合物的结构。循环伏安法和阻抗谱发现了所研究系统的电化学特性：异质电子转移速率常数、与 P. yeei 细菌相互作用的速率常数和阻抗。包含碳纳米管和二茂铁基氧化还原活性聚合物的系统被证明是最有前途的。使用混合聚合物形成的生物传感器具有高灵敏度，下限为 0。