The dual functional implants of antibacteria and osteointegration are highly demanded in orthopedic and dentistry, especially for patients who suffer from diabetes or osteoporosis simultaneously. However, there is lack of the facile and robust method to produce clinically applicable implants with this dual function although coatings possessing single function have been extensively developed. Herein, hyperbranched poly-L-lysine (HBPL) polymers were covalently immobilized onto the alkali-heat treated titanium (Ti) substrates and implants by using 3-glycidyloxypropyltrimethoxysilane (GPTMS) as the coupling agent, which displayed excellent antibacterial activity against S. aureus and E. coli with an efficiency as high as 89.4% and 92.2% in vitro, respectively. The HBPL coating also significantly promoted the adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells in vitro. Furthermore, the results of a S. aureus infection rat model in vivo ulteriorly verified that the HBPL-modified screws had good antibacterial and anti-inflammatory abilities at an early stage of implantation and better osteointegration compared with the control Ti screws.

骨科和牙科界对抗菌和骨整合的双重功能植入物提出了很高的要求，特别是对于同时患有糖尿病或骨质疏松症的患者。然而，尽管已经广泛地开发了具有单一功能的涂层，但是仍然缺乏用于生产具有这种双重功能的临床上可应用的植入物的简便且坚固的方法。在此，通过使用3-环氧丙氧基丙基三甲氧基硅烷（GPTMS）作为偶联剂，将超支化聚L-赖氨酸（HBPL）聚合物共价固定在经碱热处理的钛（Ti）基材和植入物上，该化合物对金黄色葡萄球菌具有出色的抗菌活性。和大肠杆菌，体外效率分别高达89.4％和92.2％， 分别。的HBPL还涂覆显著促进了粘合性，铺展，增殖和MC3T3-E1细胞的成骨分化的体外。此外，金黄色葡萄球菌感染大鼠模型的体内研究结果进一步证实，与对照Ti螺钉相比，HBPL修饰螺钉在植入早期具有良好的抗菌和抗炎能力，并具有更好的骨整合性。