Abstract

Protein–lipid interfaces are among the most fundamental in biology. Yet applying conventional techniques to study the biophysical attributes of these systems is challenging and has left many unknowns. For example, what is the kinetic pathway and energy landscape experienced by a polypeptide chain when in close proximity to a fluid lipid bilayer? Here we review the experimental and theoretical progress we have made in addressing this question from a single molecule perspective. Some remaining impediments are also discussed.

Graphic Abstract

中文翻译：

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在单分子水平上定量了解蛋白质-脂质双层相互作用：机遇与挑战

摘要

蛋白质-脂质界面是生物学中最基本的界面之一。然而，应用传统技术研究这些系统的生物物理属性具有挑战性，并且留下了许多未知数。例如，当靠近流体脂质双层时，多肽链经历的动力学途径和能量景观是什么？在这里，我们从单分子的角度回顾了我们在解决这个问题方面取得的实验和理论进展。还讨论了一些剩余的障碍。

图形摘要