In general, goat mammary epithelial cells (MECs) are cultured in vitro under 37 °C. We demonstrated previously that goat MECs differentiate under 37 °C although their body temperature is approximately 39 °C. This study aimed to investigate the influence of 39 °C on lactogenic differentiation of goat milk-derived MECs. The results revealed that HSP70 gene was significantly elevated at 1 h after an exposure to 39 °C but declined at 48 h thereafter. Oxidative stress status was not significantly affected by 39 °C. Expressions of CSN2, β-GALT1, α-LA, and Akt genes tended to increase after the differentiation under 39 °C. Secretion of lactose under 39 °C was not significantly lower than 37 °C. In conclusion, incubation temperature at 39 °C does not dramatically affect lactogenic function of goat milk-derived MECs.

一般来说，山羊乳腺上皮细胞 (MEC) 在 37°C 下体外培养。我们之前证明山羊 MEC 在 37°C 下分化，尽管它们的体温约为 39°C。本研究旨在研究 39°C 对山羊乳来源的 MEC 的泌乳分化的影响。结果显示，HSP70 基因在暴露于 39°C 后 1 小时显着升高，但在 48 小时后下降。氧化应激状态不受 39°C 的显着影响。CSN2、β-GALT1、α-LA和Akt基因的表达在39℃分化后有增加的趋势。39°C以下乳糖的分泌量不低于37°C。总之，39°C 的孵化温度不会显着影响山羊奶来源的 MEC 的催乳功能。