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MiR-520a-3p inhibits malignant progression of epithelial ovarian cancer by targeting SUV39H1 expression
Human Cell ( IF 4.3 ) Pub Date : 2020-11-16 , DOI: 10.1007/s13577-020-00455-2
Jingwei Li 1 , Wei Shao 1 , Junhong Zhao 1
Affiliation  

Downregulation of microRNA-520a-3p (miR-520a-3p) has been demonstrated in several cancers, and miR-520a-3p has been shown to inhibit tumor progression, indicating its potential role as a tumor suppressor. In this study, we found that miR-520a-3p was also downregulated in epithelial ovarian cancer (EOC) tissues and cell lines. Functional assays showed that ectopic expression of miR-520a-3p suppressed EOC cell proliferation, invasion, and epithelial–mesenchymal transition (EMT) and induced cell cycle arrest in vitro. Similarly, overexpression of miR-520a-3p inhibited tumor growth and metastasis in vivo. Mechanistically, suppressor of variegation 39H1 (SUV39H1) was identified as a novel target of miR-520a-3p through biomedical databases and dual-luciferase reporter assay. Subsequently, SUV39H1 was observed to be negatively regulated by miR-520a-3p at the mRNA and protein levels, and inversely correlated with miR-520a-3p expression in EOC tissues. Furthermore, overexpression of SUV39H1 reversed the suppressive effects of miR-520a-3p in EOC cells. Collectively, these results suggest that the miR-520a-3p/SUV39H1 axis may contribute to EOC cell proliferation and metastasis, revealing miR-520a-3p as a potential therapeutic target for the treatment of EOC.



中文翻译:

MiR-520a-3p 通过靶向 SUV39H1 表达抑制上皮性卵巢癌的恶性进展

microRNA-520a-3p (miR-520a-3p) 的下调已在多种癌症中得到证实,并且 miR-520a-3p 已被证明可抑制肿瘤进展,表明其作为肿瘤抑制因子的潜在作用。在这项研究中,我们发现 miR-520a-3p 在上皮性卵巢癌 (EOC) 组织和细胞系中也被下调。功能分析表明,miR-520a-3p 的异位表达抑制 EOC 细胞增殖、侵袭和上皮间质转化 (EMT),并在体外诱导细胞周期停滞。类似地,miR-520a-3p 的过表达抑制了体内肿瘤的生长和转移。从机制上讲,通过生物医学数据库和双荧光素酶报告基因检测,杂色抑制因子 39H1 (SUV39H1) 被鉴定为 miR-520a-3p 的新靶点。随后,观察到 SUV39H1 在 mRNA 和蛋白质水平上受到 miR-520a-3p 的负调控,并与 EOC 组织中的 miR-520a-3p 表达呈负相关。此外,SUV39H1 的过表达逆转了 miR-520a-3p 在 EOC 细胞中的抑制作用。总的来说,这些结果表明 miR-520a-3p/SUV39H1 轴可能有助于 EOC 细胞增殖和转移,揭示 miR-520a-3p 作为治疗 EOC 的潜在治疗靶点。

更新日期:2020-11-16
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