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Highly selective and antifouling electrochemical biosensors for sensitive MicroRNA assaying based on conducting polymer polyaniline functionalized with zwitterionic peptide
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2020-11-15 , DOI: 10.1007/s00216-020-03025-5
Dongwei Wang 1 , Jiasheng Wang 1 , Zhiling Song 2 , Ni Hui 1
Affiliation  

Ultrasensitive and low-fouling microRNA electrochemical biosensors were successfully constructed by introducing thiol-terminated antifouling molecules (peptide sequence, polyethylene glycol, or mercapto alcohol) onto the surface of polyaniline-modified electrodes. For the three kinds of antifouling materials investigated, the newly designed and synthesized peptide exhibited superior antifouling ability to others, and it could effectively reduce the nonspecific adsorption of proteins and even prevent the fouling effect of serum. Compared with microRNA biosensors without antifouling capability, or those modified with polyethylene glycol or mercapto alcohol, the biosensor modified with the designed zwitterionic peptide showed the highest specificity for single-base mismatch, three-base mismatch, and completely complementary microRNAs. Most interestingly, the experimental results indicated that the introduction of antifouling molecules to the sensing interfaces did not significantly change the sensitivity of the biosensor. The strategy of constructing antifouling biosensors based on newly synthesized zwitterionic peptides and conducting polymers can be promisingly extended to the development of other electrochemical sensors and biosensors without encountering biofouling.



中文翻译:

基于两性离子肽功能化的导电聚合物聚苯胺的高选择性和防污电化学生物传感器,用于灵敏的 MicroRNA 检测

通过将硫醇封端的防污分子(肽序列、聚乙二醇或巯基醇)引入聚苯胺修饰电极表面,成功构建了超灵敏、低污染的 microRNA 电化学生物传感器。对于所研究的三种防污材料,新设计合成的多肽表现出优于其他产品的防污能力,可有效减少蛋白质的非特异性吸附,甚至可以防止血清的污染作用。与不具有防污能力的microRNA生物传感器或经聚乙二醇或巯基醇修饰的microRNA生物传感器相比,用设计的两性离子肽修饰的生物传感器对单碱基错配、三碱基错配和完全互补的microRNAs表现出最高的特异性。最有趣的是,实验结果表明,将防污分子引入传感界面并没有显着改变生物传感器的灵敏度。基于新合成的两性离子肽和导电聚合物构建防污生物传感器的策略可以有希望地扩展到其他电化学传感器和生物传感器的开发,而不会遇到生物污垢。

更新日期:2020-11-16
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