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A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage
Analyst ( IF 4.2 ) Pub Date : 2020-11-14 , DOI: 10.1039/d0an01240h
Gaoqing Fu 1, 2, 3, 4 , Guoxing Yin 4, 5, 6, 7 , Tingting Niu 1, 2, 3, 4 , Wei Wu 1, 2, 3, 4 , Hui Han 1, 2, 3, 4 , Haimin Chen 1, 2, 3, 4 , Peng Yin 4, 5, 6, 7
Affiliation  

A sensitive fluorescent probe (E)-4-(3-(benzo[d]thiazol-2-yl)-4-hydroxy-5-methylstyryl)-1-methylpyridin-1-ium iodide (HBTMP) for the monitoring of pH in mitochondria was rationally exploited. This novel probe exhibited remarkable pH-dependent behavior in the linear range of 5.5–8.0, along with a pKa value of 6.829 ± 0.02627. A large Stokes shift of 205 nm was obtained. This fluorescent probe demonstrated good biocompatibility and high sensitivity for detecting the dynamic changes in mitochondrial pH in living cells and zebrafish. The results of the CCCP (m-chlorophenyl hydrazone) treatment experiment indicated that the probe can effectively monitor changes in mitochondrial pH caused by cell damage.

中文翻译:

一种新型比例荧光探针,可检测细胞损伤期间线粒体的pH动态

用于监测pH值的灵敏荧光探针(E)-4-(3-(苯并[ d ]噻唑-2-基)-4-羟基-5-甲基苯乙烯基)-1-甲基吡啶-1-碘化物(HBTMP)线粒体被合理利用。这种新颖的探头中的5.5-8.0线性范围显示出显着的pH依赖性的行为,与AP沿ķ一个的6.829±0.02627值。获得205nm的大斯托克斯位移。该荧光探针显示出良好的生物相容性和高灵敏度,可检测活细胞和斑马鱼中线粒体pH的动态变化。的CCCP的结果(氯苯基腙)治疗实验表明,该探头可有效地监控所引起的细胞损伤线粒体pH变化。
更新日期:2020-11-15
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