Phase imaging microscopy with incoherent object illumination is convenient and affordable for biomedical research and clinics since it provides easy integration with a variety of bright-field optical microscopes. We report the design of a new hyperspectral imaging system based on a combination of a spatial light modulator (SLM) and an acousto-optic tunable filter (AOTF) for phase imaging microscopy. Contrast of phase-only objects originates from matched spectral and spatial filtering performed by the SLM and the AOTF located in Fourier-conjugate optical planes in the back-end of the optical system. The system is designed as an add-on to a standard optical microscope with incoherent diascopic sample illumination.

中文翻译：

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基于AOTF的高光谱成像相显微镜

具有非相干物体照明的相位成像显微镜对于生物医学研究和临床而言既方便又经济，因为它可以轻松地与各种明场光学显微镜集成。我们报告了基于空间光调制器（SLM）和声光可调滤光片（AOTF）相成像显微镜相结合的新的高光谱成像系统的设计。仅相位对象的对比度源自SLM和位于光学系统后端的傅立叶共轭光学平面中的AOTF执行的匹配光谱和空间滤波。该系统被设计为具有不相干的透明样品照明的标准光学显微镜的附件。