The wound-healing assay is a simple but effective tool for studying collective cell migration (CCM) that is widely used in biophysical studies and high-throughput screening. However, conventional imaging and analysis methods only address two-dimensional (2D) properties in a wound healing assay, such as gap closure rate. This is unfortunate because biological cells are complex 3D structures, and their dynamics provide significant information about cell physiology. Here, we presented 3D label-free imaging for wound healing assays and investigated the 3D dynamics of CCM using optical diffraction tomography. High-resolution subcellular structures as well as their collective dynamics were imaged and analyzed quantitatively.

中文翻译：

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伤口愈合过程中迁移细胞的三维无标记成像和定量

伤口愈合测定法是研究集体细胞迁移（CCM）的简单但有效的工具，广泛用于生物物理研究和高通量筛选。但是，常规的成像和分析方法仅解决伤口愈合测定中的二维（2D）特性，例如间隙闭合率。这是不幸的，因为生物细胞是复杂的3D结构，并且其动力学提供了有关细胞生理的重要信息。在这里，我们提出了用于伤口愈合测定的无3D标签成像技术，并使用光学衍射层析成像技术研究了CCM的3D动力学。高分辨率的亚细胞结构及其集体动力学被成像和定量分析。