Retinal microvasculature and the retinal pigment epithelium (RPE) play vital roles in maintaining the health and metabolic activity of the eye. Visualization of these retina structures is essential for pre-clinical studies of vision-robbing diseases, such as age-related macular degeneration (AMD). We have developed a quantitative multi-contrast polarization diversity OCT and angiography (QMC-PD-OCTA) system for imaging and visualizing pigment in the RPE using degree of polarization uniformity (DOPU), along with flow in the retinal capillaries using OCT angiography (OCTA). An adaptive DOPU averaging kernel was developed to increase quantifiable values from visual data, and QMC en face images permit simultaneous visualization of vessel location, depth, melanin region thickness, and mean DOPU values, allowing rapid identification and differentiation of disease symptoms. The retina of five different mice strains were measured in vivo, with results demonstrating potential for pre-clinical studies of retinal disorders.

中文翻译：

---

采用偏振分集光学相干断层扫描和血管造影进行定量多对比小鼠体内成像

视网膜微血管和视网膜色素上皮（RPE）在维持眼睛的健康和代谢活动中发挥着至关重要的作用。这些视网膜结构的可视化对于视力丧失疾病（例如年龄相关性黄斑变性（AMD））的临床前研究至关重要。我们开发了一种定量多对比偏振分集 OCT 和血管造影 (QMC-PD-OCTA) 系统，用于使用偏振均匀度 (DOPU) 对 RPE 中的色素进行成像和可视化，并使用 OCT 血管造影 (OCTA) 对视网膜毛细血管中的血流进行成像和可视化。 ）。开发了自适应 DOPU 平均内核来增加视觉数据的可量化值，QMC面部图像允许同时可视化血管位置、深度、黑色素区域厚度和平均 DOPU 值，从而可以快速识别和区分疾病症状。对五种不同小鼠品系的视网膜进行了体内测量，结果证明了视网膜疾病临床前研究的潜力。