Glaucoma filtration surgery (GFS) commonly fails due to excessive fibrosis. As collagen structure aberrations is implicated in adverse fibrotic progression, this study aims to uncover collagen organization alterations during postoperative scarring. Via quantitative second harmonic generation/ two photon excitation multiphoton imaging, we reveal the scar development and phenotype in the mouse model of conjunctival scarring. We also show that multiphoton imaging corroborated the collagen ultrastructure anomaly characteristic of the SPARC-/- mouse postoperative conjunctiva. These data improve our understanding of postoperative conjunctival scarring and further enhance the utility of this model for the development of anti-fibrotic therapeutics for GFS.

中文翻译：

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多光子显微镜评估结膜术后胶原组织进行性改变

青光眼滤过手术（GFS）通常由于过度的纤维化而失败。由于胶原蛋白结构异常与不良的纤维化进展有关，因此本研究旨在揭示术后瘢痕形成过程中胶原蛋白组织的变化。通过定量二次谐波产生/两光子激发多光子成像，我们揭示了结膜瘢痕小鼠模型中的瘢痕形成和表型。我们还显示，多光子成像证实了SPARC-/-小鼠术后结膜的胶原超微结构异常特征。这些数据改善了我们对术后结膜瘢痕形成的了解，并进一步增强了该模型在开发GFS抗纤维化治疗剂中的实用性。