Optical diffraction tomography (ODT) is an indispensable tool for studying objects in three dimensions. Until now, ODT has been limited to coherent light because spatial phase information is required to solve the inverse scattering problem. We introduce a method that enables ODT to be applied to imaging incoherent contrast mechanisms such as fluorescent emission. Our strategy mimics the coherent scattering process with two spatially coherent illumination beams. The interferometric illumination pattern encodes spatial phase in temporal variations of the fluorescent emission, thereby allowing incoherent fluorescent emission to mimic the behavior of coherent illumination. The temporal variations permit recovery of the spatial distribution of fluorescent emission with an inverse scattering model. Simulations and experiments demonstrate isotropic resolution in the 3D reconstruction of a fluorescent object.

中文翻译：

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单像素荧光衍射断层扫描

光学衍射断层扫描 (ODT) 是研究三维物体不可或缺的工具。到目前为止，ODT 仅限于相干光，因为需要空间相位信息来解决逆散射问题。我们介绍了一种方法，使 ODT 能够应用于成像非相干对比度机制，如荧光发射。我们的策略模拟了两个空间相干照明光束的相干散射过程。干涉照明模式在荧光发射的时间变化中编码空间相位，从而允许非相干荧光发射模仿相干照明的行为。时间变化允许使用逆散射模型恢复荧光发射的空间分布。