Abstract

Polymer science offers a great insight and a new research dimension for biomedical applications. The synthesis of polymeric materials by the physical ways provides several advantages over the conventional chemical methods. It is though expansive but less toxic, stable, and efficiently reproducible. In the present report, electrospinning was used for bio-composite preparation. The bio-composite was developed using polyvinyl alcohol (PVA) and curcumin. The electrospun fiber bio-composite were analyzed for antibacterial activity, bacterial filtration capability, and endotoxin elimination. The bio-composite was analyzed for physical structure and properties using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray analysis (EDX), and Fourier Transform Infra-Red spectroscopy (FT-IR). PVA solely was not able to exhibit any of the antibacterial or endotoxin removal properties. However, the curcumin-based bio-composite was found to be bactericidal and endotoxin eliminator. The bio-composite was able to remove 100% of endotoxin and nearly 100% of the bacterial cells. The endotoxin removal properties of bio-composite were found to be excellent fit under Langmuir curve with a R² value of 0.98. Additionally, the effect of bio-composite was also studied over protein content in the sample and L-asparaginase activity. However, the effect observed was negligible.

摘要

聚合物科学为生物医学应用提供了深刻的见解和新的研究维度。与传统化学方法相比，通过物理方式合成聚合物材料具有多种优势。它虽然膨胀但毒性较小，稳定且可有效再现。在本报告中，静电纺丝用于生物复合材料的制备。这种生物复合材料是使用聚乙烯醇 (PVA) 和姜黄素开发的。分析了电纺纤维生物复合材料的抗菌活性、细菌过滤能力和内毒素消除能力。使用扫描电子显微镜 (SEM)、能量色散 X 射线分析 (EDX) 和傅里叶变换红外光谱 (FT-IR) 分析生物复合材料的物理结构和性能。仅 PVA 不能表现出任何抗菌或内毒素去除特性。然而，发现基于姜黄素的生物复合材料具有杀菌和内毒素消除作用。这种生物复合材料能够去除 100% 的内毒素和近 100% 的细菌细胞。发现生物复合材料的内毒素去除特性在 Langmuir 曲线下具有很好的拟合²值为 0.98。此外，还研究了生物复合材料对样品中蛋白质含量和 L-天冬酰胺酶活性的影响。然而，观察到的影响可以忽略不计。