Abstract Serratia liquefaciens is a spoilage microorganism of relevance in the dairy industry because it is psychrotrophic, able to form biofilm, and produces thermoresistant proteases and lipases. Phenolic compounds and furanones have been studied as inhibitors of biofilm formation. In this study, the potential of the pulp phenolic extract of Eugenia uniflora L. orange fruits, also called pitanga, and furanone C30 on the inhibition of biofilm formation by S. liquefaciens L53 and the susceptibility to different antimicrobials were evaluated. The pulp phenolic extract of pitanga had a high total phenolic content, being mainly composed of glycosylated quercetins and ellagitannins. Sub-inhibitory concentrations of this extract and furanone reduced biofilm formation by S. liquefaciens on polystyrene and the amount of polysaccharides, proteins and extracellular DNA in the biofilms. These biofilms were also more susceptible to kanamycin. The combinations of furanone with phenolic extract of pitanga or kanamycin showed a synergistic effect with total growth inhibition of S. liquefaciens.

中文翻译：

---

Eugenia uniflora L. 和呋喃酮的酚类提取物减少了液化沙雷氏菌的生物膜形成并增加了其对抗菌剂的敏感性

摘要 液化沙雷氏菌是一种与乳制品行业相关的腐败微生物，因为它具有嗜冷性，能够形成生物膜，并产生耐热蛋白酶和脂肪酶。酚类化合物和呋喃酮已被研究作为生物膜形成的抑制剂。在这项研究中，评估了 Eugenia uniflora L. 橙色果实（也称为 pitanga）和呋喃酮 C30 的果肉酚类提取物对抑制 S. liquefaciens L53 生物膜形成的潜力以及对不同抗菌剂的敏感性。皮坦加果肉酚类提取物的总酚含量较高，主要由糖基化槲皮素和鞣花单宁组成。这种提取物和呋喃酮的亚抑制浓度减少了液化链球菌对聚苯乙烯的生物膜形成和多糖的数量，生物膜中的蛋白质和细胞外 DNA。这些生物膜也对卡那霉素更敏感。呋喃酮与 pitanga 或卡那霉素的酚类提取物的组合显示出对液化链球菌的总生长抑制的协同作用。