A rich of 3,4‐seco‐lupane triterpenoids including chiisanoside (CSS), divaroside (DVS), sessiloside‐A1 (SSA) and chiisanogenin (CSG) were isolated from the ethanol extract of the leaves of Acanthopanax sessiliflorus. On the basis of previous studies, this article focused on four important components of 3,4‐seco‐lupane triterpenoids in Acanthopanax sessiliflorus leaves and explored their protective effects against aconitine‐induced cardiomyocyte injury and their molecular mechanisms. The results showed that pretreatment with 3,4‐seco‐lupane triterpenoids could effectively increase cell viability, reduce CK‐MB and LDH activities, reduce ROS production, maintain calcium concentration balance, and inhibit apoptosis, with divaroside having the best effect. In addition, Western blot results showed that divaroside down‐regulated Cleaved caspase‐3 and Bax and up‐regulated Bcl‐2 expression through activating the PI3 K/AKT pathway. However, the LY294002 inhibitor reversed this situation. This suggests that 3,4‐seco‐lupane triterpenoids may be a new hotspot for potential myocardial protective drugs research.

中文翻译：

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刺五加叶中的 3,4-seco-lupane 三萜类化合物对心肌的潜在保护作用

从无柄刺五加叶的乙醇提取物中分离出丰富的 3,4-seco-lupane 三萜类化合物，包括 chiisanoside (CSS)、divaroside (DVS)、sessiloside-A1 (SSA) 和 chiisanogenin (CSG)。本文在前人研究的基础上，重点研究了刺五加叶中 3,4-seco-lupane 三萜类化合物的 4 个重要成分，探讨了它们对乌头碱致心肌细胞损伤的保护作用及其分子机制。结果表明，用3,4-seco-lupane三萜类化合物预处理可有效提高细胞活力，降低CK-MB和LDH活性，减少ROS的产生，维持钙浓度平衡，抑制细胞凋亡，其中以二果苷为最佳。此外，蛋白质印迹结果表明，二甘草苷通过激活 PI3 K/AKT 通路下调 Cleaved caspase-3 和 Bax 并上调 Bcl-2 表达。然而，LY294002 抑制剂扭转了这种情况。这表明 3,4-seco-lupane 三萜类化合物可能成为潜在的心肌保护药物研究的新热点。