In this study, six vertical flow constructed wetlands (VFCWs) planted with Phragmites australis were operated at different aeration times (4 h day⁻¹ and 8 h day⁻¹), aeration modes (continuous and intermittent), and arbuscular mycorrhizal fungi (AMF) inoculation treatments (inoculation with Rhizophagus intraradices and no inoculation) to explore the effects of different aeration strategies on the formation of arbuscular mycorrhiza under a flooded state in VFCWs. In addition, these VFCWs were further used to treat copper oxide nanoparticle (CuO-NP) wastewater to evaluate the correlations among aeration, colonization, growth, and CuO-NP removal. The highest AMF 28S copy number (1.99×10⁵) and colonization in reed roots, with values of 67%, 21%, and 1% for frequency (F%), intensity (M%), and arbuscule abundance (A%), were observed in the treatment with intermittent aeration for 4 h day⁻¹. Aeration significantly increased the dissolved oxygen (DO) concentration and AMF colonization in VFCWs, thereby promoting plant growth and the purification of the CuO-NPs. However, excessive and continuous aeration had little positive effect on AMF colonization. This study provides a theoretical basis for the application of AMF for improving pollutant removal performance in constructed wetlands.

在这项研究中，种植了芦苇的6 个垂直流人工湿地 (VFCW)在不同的曝气时间（4 h day ^-1和 8 h day ^-1）、曝气模式（连续和间歇）和丛枝菌根真菌（AMF）下运行) 接种处理（接种根内根瘤菌和不接种），以探讨不同曝气策略对 VFCWs 淹水状态下丛枝菌根形成的影响。此外，这些 VFCW 进一步用于处理氧化铜纳米颗粒 (CuO-NP) 废水，以评估曝气、定植、生长和 CuO-NP 去除之间的相关性。最高 AMF 28S 拷贝数（1.99×10 ⁵) 和芦苇根的定植，频率 (F%)、强度 (M%) 和丛枝丰度 (A%) 的值分别为 67%、21% 和 1%，在间歇通气 4 h 天^-1。曝气显着增加了 VFCWs 中的溶解氧 (DO) 浓度和 AMF 定植，从而促进植物生长和 CuO-NPs 的纯化。然而，过度和连续曝气对 AMF 定植几乎没有积极影响。该研究为应用AMF提高人工湿地污染物去除性能提供了理论依据。