Rhizobium is a plant growth-promoting bacteria, generally involved in nitrogen fixation and promotes growth in plants under abiotic-stressed conditions such as salinity. The present study investigates the significance of Rhizobium application in alleviation of salt stress in chickpea by increasing cell viability, stomatal movement, photosynthetic pigment and protein content, nitrate reductase, carbonic anhydrase as well as enzymatic and non-enzymatic antioxidant activities. Healthy and viable seeds were inoculated with Rhizobium before sowing. Salt treatment was given in terms of NaCl (50 or 150 mM) to the plants through soil at 20 days after sowing. High NaCl level (150 mM) reduced the growth attributes, pigment as well as soluble protein content, altered stomatal behaviour, reduced cell viability and enhanced the formation of superoxide radicals and other reactive oxygen species in foliage. Moreover, Rhizobium inoculation improved the mineral uptake, reduced electrolyte leakage which directly influences photosynthesis and improved yield attributes in the salt-treated chickpea plants. Therefore, Rhizobium could be applied to chickpea plants for efficient growth under salt stress.

中文翻译：

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接种根瘤菌通过调节 Cicer arietinum L 的生长特性、生理和生化特性、气孔活动和抗氧化防御来减轻盐分胁迫。

根瘤菌是一种促进植物生长的细菌，通常参与固氮作用，并在盐度等非生物胁迫条件下促进植物生长。本研究通过增加细胞活力、气孔运动、光合色素和蛋白质含量、硝酸还原酶、碳酸酐酶以及酶促和非酶促抗氧化活性，研究根瘤菌在减轻鹰嘴豆盐胁迫中的意义。健康和有活力的种子在播种前接种根瘤菌。在播种后 20 天通过土壤对植物进行 NaCl（50 或 150 mM）盐处理。高 NaCl 水平 (150 mM) 降低了生长属性、色素和可溶性蛋白质含量，改变了气孔行为，降低细胞活力并增强超氧自由基和其他活性氧在叶子中的形成。此外，根瘤菌接种提高了矿物质吸收，减少了直接影响光合作用的电解质泄漏，并提高了盐处理鹰嘴豆植物的产量属性。因此，根瘤菌可用于鹰嘴豆植物在盐胁迫下的高效生长。