The present study was aimed to develop a most effective extender for long term storage of Labrador dog semen at 4^oC. Tris-citric acid-fructose was supplemented with egg yolk (TEY), egg yolk plasma (TEYP), low density lipoproteins (TLDL) and coconut water (TCW) @10, 15 and 20; 10, 15 and 20; 11-15; and 25, 50 and 75 percent, respectively. Extended semen was stored at 4^oC and analyzed for sperm attributes and lipid peroxidation. Values for motility, viability and plasma membrane integrity remained significantly (p < 0.05) higher in 15% TEY, 15% TEYP, 13% TLDL and 25%/50% TCW from 0 hr to 72 hrs of storage. It indicated superiority of 15% TEY, 15% TEYP, 13% TLDL and 25% /50% TCW over other concentrations for storage of Labrador dog semen at 4^oC. A significant (p < 0.05) decline in motility, viability and membrane integrity was observed from 0-72 hrs of preservation in all extenders, but sperm attributes were still >50% at 72 hrs of preservation. Decline was comparatively less in 15% TEY, 15% TEYP, 25%/50% TCW and 13% TLDL compared to other concentrations. Values for acrosome integrity also differ significantly (p > 0.05) among different concentrations of extenders except TEY. Lipid peroxidation did not vary among different extenders except TEY. In conclusion, both 15% TEY and 15% TEYP were better than 13% TLDL and 25% TCW. TEY extender may be substituted with TEYP for preservation of dog semen at 4°C and further interventions may improve TLDL and TCW extenders.

中文翻译：

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评估和比较四种实验室制造的tris碱补充剂，用于在4ºC保存拉布拉多犬的狗精液

本研究旨在开发一种最有效的延长剂，用于在4 ^o C下长期保存拉布拉多犬精液。三柠檬酸果糖补充了蛋黄（TEY），蛋黄血浆（TEYP），低密度脂蛋白（ TLDL）和椰子水（TCW）@ 10、15和20；10、15和20；11-15; 和25％，50％和75％。延长精液储存在4 ^o C并分析精子属性和脂质过氧化作用。从0小时到72小时，在15％TEY，15％TEYP，13％TLDL和25％/ 50％TCW中，运动性，生存力和质膜完整性的值仍显着（p <0.05）高。这表明在4 ^o L储存拉布拉多犬精液比其他浓度有15％的TEY，15％的TEYP，13％TLDL和25％/ 50％的TCW优势^。在所有补充剂中，从保存0-72小时观察到运动性，生存力和膜完整性显着（p <0.05）下降，但是在保存72小时时，精子属性仍> 50％。与其他浓度相比，15％TEYP，15％TEYP，25％/ 50％TCW和13％TLDL的下降相对较小。除TEY外，不同浓度的补充剂中顶体完整性的值也存在显着差异（p> 0.05）。除TEY外，不同补充剂之间的脂质过氧化作用均无差异。总之，15％的TEY和15％的TEYP均优于13％的TLDL和25％的TCW。TEY补充剂可以用TEYP代替，以在4°C保存狗精液，进一步的干预可以改善TLDL和TCW补充剂。